Rethinking Amino Acid Nutrition of Black Soldier Fly Larvae (

Rearing black soldier fly larvae (BSFL) in order to utilize biogenic materials is of increasing interest in the context of sustainable animal production. However, little is known about the amino acid (AA) requirements of this animal species. Therefore, a feeding experiment with BSFL was conducted, in which lysine, methionine, cysteine, arginine, phenylalanine, or histidine was reduced by 65% or methionine was increased by 65%. Reductions in single AAs in the substrate did not negatively impact BSFL growth, while the addition of methionine improved the growth performance, indicating that methionine was a limiting nutritional factor for maximal productivity. Differences in dietary AA profiles had no impact on the AA profile of the BSFL biomass. However, balance calculations for individual AAs indicated that the substrate microbiome was capable of synthesizing and balancing those AAs for microbial protein synthesis. While both the BSFL and microbiome utilized all free AAs, suggesting that supplemental AAs can be effective additives in BSFL production, this microbial AA synthesis avoided the negative performance impacts of BSFL provoked by severe AA reductions. The quantification of these effects suggested that the microbiome could add up to 35% to the overall AA supply in order to overcome AA deficiency. This effect may not necessarily ensure maximal BSFL productivity, as demonstrated by the extra addition of methionine to the substrate. Our research indicated that BSFL nutrition should consider interactions between substrate composition and microbial activity.