Evidence map›Paper›PMID 39589701›Full record

ArticleApplied biochemistry and biotechnology2025

Different Effects of Deglycosylation on the Lactose Sensing Ability of Mesophilic and Thermophilic Cellobiose Dehydrogenases.

Yaohong Ma, Yunlong Xue, Xingbao Wang, Yue Shao, Xiaozhen Huang, Zhenyu Zhang, Sirong Zhu, Weili Gong

Abstract read
PubMed Publisher
In one paragraph

Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yaohong Ma *Shandong Provincial Key Laboratory of Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789, Jingshi East Road, Licheng District, Jinan, 250103, Shandong, China.
Yunlong Xue *Shandong Provincial Key Laboratory of Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789, Jingshi East Road, Licheng District, Jinan, 250103, Shandong, China.
Xingbao WangShandong Provincial Key Laboratory of Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789, Jingshi East Road, Licheng District, Jinan, 250103, Shandong, China.
Yue ShaoShandong Provincial Key Laboratory of Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789, Jingshi East Road, Licheng District, Jinan, 250103, Shandong, China.
Xiaozhen HuangShandong Provincial Key Laboratory of Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789, Jingshi East Road, Licheng District, Jinan, 250103, Shandong, China.
Zhenyu ZhangShandong Provincial Key Laboratory of Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789, Jingshi East Road, Licheng District, Jinan, 250103, Shandong, China.
Sirong ZhuShandong Provincial Key Laboratory of Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789, Jingshi East Road, Licheng District, Jinan, 250103, Shandong, China.
Weili GongShandong Provincial Key Laboratory of Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), No. 28789, Jingshi East Road, Licheng District, Jinan, 250103, Shandong, China. 15264110812@163.com.

Funding

the Key innovation Project of Qilu University of Technology (Shandong Academy of Sciences) 2022JBZ01-06the National Key R&D Program of China 2021YFB3201200the National Key R&D Program of China 2021YFB3201203the National Natural Science Foundation of China 32101222
6 · The paper itself

Abstract

The development of an efficient lactose biosensor employing cellobiose dehydrogenases (CDHs) for monitoring and precise control of the lactose levels in dairy-based products is extremely important for the health of lactose-intolerant population. In this study, the mesophilic (Nc_CDH) and thermophilic (Ct_CDH-A, Ct_CDH-B) CDHs were successfully obtained by heterologous expression and treated with α-1,2-mannosidase and endoglycosidase H to prepare the deglycosylated forms (Nc_dCDH, Ct_dCDH-A, and Ct_dCDH-B); then, the effects of deglycosylation on the catalytic activity in solution and electrochemical performance on electrodes for lactose detection were systematically investigated. In solution, Nc_dCDH was more stable and had a higher V

Indexed as

Biosensing TechniquesCarbohydrate DehydrogenasesLactoseEnzymes, ImmobilizedEnzyme StabilityGlycosylationNanotubes, CarbonTemperatureCarbohydrate Dehydrogenasescellobiose-quinone oxidoreductaseEnzymes, ImmobilizedLactoseNanotubes, CarbonBiosensorDeglycosylationElectrochemical performanceLactoseMesophilic and thermophilic cellobiose dehydrogenase

Identifiers

PMID39589701

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.