Evidence map›Paper›PMID 39589399›Full record

ArticleThe Journal of general virology2024

African lineage 1a West Nile virus isolated from crocodiles exhibits low neuroinvasiveness in mice.

Hiroko Kobayashi, Herman Chambaro, Koshiro Tabata, Takuma Ariizumi, Wallaya Phongphaew, Kunda Ndashe, Joseph Ndebe, Paul Fandamu, Shintaro Kobayashi, Naoto Ito and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of general virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Hiroko KobayashiDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Herman ChambaroDepartment of Veterinary Services, Ministry of Fisheries and Livestock, Lusaka, Zambia.
Koshiro TabataInstitute for Vaccine Research and Development, Hokkaido University, Sapporo, Japan.
Takuma AriizumiDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Wallaya PhongphaewDepartment of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
Kunda NdasheDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
Joseph NdebeDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
Paul FandamuDepartment of Veterinary Services, Ministry of Fisheries and Livestock, Lusaka, Zambia.
Shintaro KobayashiInstitute for Vaccine Research and Development, Hokkaido University, Sapporo, Japan.
Naoto ItoLaboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Michihito SasakiDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Bernard M Hang'ombeDepartment of Para-clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
Edgar SimulunduMacha Research Trust, Choma, Zambia.
Yasuko OrbaDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Hirofumi SawaInternational Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

West Nile virus (WNV) is a mosquito-borne flavivirus that causes encephalitis in humans and infects crocodiles, resulting in rashes and neurological signs. In Zambia, two distinct lineages of WNV have been detected in neighbouring areas: lineage 2 in mosquitoes and lineage 1a in farmed crocodiles. Considering the risk of direct or vector-mediated WNV transmission from crocodiles to mammals, it is necessary to elucidate the pathogenicity of WNV strains derived from crocodiles. In this study, WNV was successfully isolated from naturally infected farmed crocodiles (Croc110/2019/1/ZM, Croc110). We then investigated its proliferation and pathogenicity in mice in comparison with a WNV isolate from mosquitoes in Zambia (Zmq16) and two reference strains, including one highly pathogenic (NY99) and one low pathogenic (Eg101) strain. Although viral proliferation in Vero and mammalian neuronal cells was comparable among the strains, Croc110 exhibited low cell-to-cell transmission efficiency.

Indexed as

Alligators and CrocodilesMice, Inbred C57BLWest Nile FeverWest Nile virusAnimalsChlorocebus aethiopsFemaleMiceVero CellsVirulenceZambiacrocodileflaviviruspathogenicity in micevirus isolationWest Nile virus

Identifiers

PMID39589399
PMCPMC11652736

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.