Evidence map›Paper›PMID 39588857›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2025

Bacteriophage-Activated DNAzyme Hydrogels Combined with Machine Learning Enable Point-of-Use Colorimetric Detection of Escherichia coli.

Hannah Mann, Shadman Khan, Akansha Prasad, Fereshteh Bayat, Jimmy Gu, Kyle Jackson, Yingfu Li, Zeinab Hosseinidoust, Tohid F Didar, Carlos D M Filipe

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
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  6. Article
  7. Review
  8. Micro- and Nano-Bots for Infection Control.Advanced materials (Deerfield Beach, Fla.) · 2025
    Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hannah MannDepartment of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0009-0003-9595-0569
Shadman KhanSchool of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0002-5088-3441
Akansha PrasadSchool of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0002-2636-5097
Fereshteh BayatSchool of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0002-8868-1650
Jimmy GuDepartment of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0003-4313-9523
Kyle JacksonDepartment of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0002-1824-3604
Yingfu LiDepartment of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0002-7533-6743
Zeinab HosseinidoustDepartment of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0001-9261-7611
Tohid F DidarSchool of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0002-8757-8002
Carlos D M FilipeDepartment of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.ORCID 0000-0002-7410-3323

Funding

Canada Research Chairs ProgramGovernment of OntarioNatural Sciences and Engineering Research Council of CanadaToyota Tsusho Canada
6 · The paper itself

Abstract

Developing cost-effective, consumer-accessible platforms for point-of-use environmental and clinical pathogen testing is a priority, to reduce reliance on laborious, time-consuming culturing approaches. Unfortunately, a system offering ultrasensitive detection capabilities in a form that requires little auxiliary equipment or training has remained elusive. Here, a colorimetric DNAzyme-crosslinked hydrogel sensor is presented. In the presence of a target pathogen, DNAzyme cleavage results in hydrogel dissolution, yielding the release of entrapped gold nanoparticles in a manner visible to the naked eye. Recognizing that Escherichia coli holds high relevance within both environmental and clinical environments, an E. coli-responsive DNAzyme is incorporated into this platform. Through the optimization of the hydrogel polymerization process and the discovery of bacteriophage-induced DNAzyme signal amplification, 10

Indexed as

BacteriophagesColorimetryDNA, CatalyticEscherichia coliHydrogelsMachine LearningPoint-of-Care SystemsBiosensing TechniquesGoldHumansMetal NanoparticlesDNA, CatalyticGoldHydrogelsclinical diagnosticscolorimetric sensorcontamination sensingDNAzymeE. coli detectionhydrogel sensormachine learning

Identifiers

PMID39588857
PMCPMC11756048

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.