Evidence map›Paper›PMID 39587642›Full record

ArticleBiology of sex differences2024

3D in vitro modelling of post-partum cardiovascular health reveals unique characteristics and signatures following hypertensive disorders in pregnancy.

Clara Liu Chung Ming, Dillan Pienaar, Sahar Ghorbanpour, Hao Chen, Lynne Margaret Roberts, Louise Cole, Kristine C McGrath, Matthew P Padula, Amanda Henry, Carmine Gentile and 1 more

Abstract read
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Article in Biology of sex differences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Generation and applications of cardiac spheroids.npj biomedical innovations · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Clara Liu Chung MingSchool of Biomedical Engineering, Faculty of Engineering and Information Technology, University of Technology Sydney, Sydney, NSW, Australia.
Dillan PienaarSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Sahar GhorbanpourSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Hao ChenSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Lynne Margaret RobertsDepartment of Women's and Children's Health, St. George Hospital, Sydney, NSW, Australia.
Louise ColeAustralian Institute of Microbiology and Infection, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Kristine C McGrathSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Matthew P PadulaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Amanda HenryDepartment of Women's and Children's Health, St. George Hospital, Sydney, NSW, Australia.
Carmine GentileSchool of Biomedical Engineering, Faculty of Engineering and Information Technology, University of Technology Sydney, Sydney, NSW, Australia. carmine.gentile@uts.edu.au.
Lana McClementsSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia. lana.mcclements@uts.edu.au.

Funding

National Heart Foundation of Australia 106628Sydney Partnership of Health, Education, Research and Enterprise (SPHERE) Cardiac and Vascular Health Clinical Academic Group
6 · The paper itself

Abstract

backgroundHypertensive disorders of pregnancy (HDP) affect 2-8% of pregnancies and are associated postpartum with increased cardiovascular disease (CVD) risk, although mechanisms are poorly understood.

methodsHuman induced pluripotent stem cells (iPSC)-derived cardiomyocytes, cardiac fibroblasts and coronary artery endothelial cells were cocultured to form cardiac spheroids (CSs) in collagen type-1 hydrogels containing 10% patient plasma collected five years postpartum [n = 5 per group: normotensive control, gestational hypertension (GH) and preeclampsia (PE)]. Plasma-treated CSs were assessed for cell viability and contractile function and subjected to immunofluorescence staining and imaging. A quantitative proteomic analysis of plasma samples was conducted (controls n = 21; GH n = 5; PE n = 12).

resultsContraction frequency (CF) was increased in PE-treated CSs (CF: 45.5 ± 3.4 contractions/minute, p < 0.001) and GH-treated CSs (CF: 45.7 ± 4.0 contractions/minute, p < 0.001), compared to controls (CF = 21.8 ± 2.6 contractions/min). Only PE-treated CSs presented significantly increased fractional shortening (FS) % (9.95 ± 1.8%, p < 0.05) compared to controls (3.7 ± 1.1%). GH-treated CSs showed a reduction in cell viability (p < 0.05) and an increase in α-SMA expression (p < 0.05). Proteomics analyses identified twenty differentially abundant proteins, with hemoglobin A2 being the only protein perturbed in both GH and PE versus control plasma (p < 0.05).

conclusionsThe innovative patient-relevant CS platforms led to the discovery of biomarkers/targets linked to cell death signaling and cardiac remodeling in GH-induced CVD and vascular/endothelial cell dysfunction in PE-induced CVD.

Indexed as

Hypertension, Pregnancy-InducedAdultCells, CulturedEndothelial CellsFemaleFibroblastsHumansInduced Pluripotent Stem CellsMyocytes, CardiacPostpartum PeriodPregnancyBiomarkersCardiovascular diseaseHypertensive disorders of pregnancyPost-partumPregnancyProteomics

Identifiers

PMID39587642
PMCPMC11587612

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.