Evidence map›Paper›PMID 39587073›Full record

ArticleNature communications2024

Autoantibodies immuno-mechanically modulate platelet contractile force and bleeding risk.

Oluwamayokun Oshinowo, Renee Copeland, Anamika Patel, Nina Shaver, Meredith E Fay, Rebecca Jeltuhin, Yijin Xiang, Christina Caruso, Adiya E Otumala, Sarah Hernandez and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Oluwamayokun OshinowoThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.ORCID 0000-0002-0077-2989
Renee CopelandThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Anamika PatelDepartment of Biochemistry, Emory University School of Medicine, Emory University, Atlanta, GA, USA.ORCID 0000-0003-4760-9019
Nina ShaverThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Meredith E FayThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Rebecca JeltuhinThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Yijin XiangDepartment of Pediatrics, Aflac Cancer Center and Blood Disorders Service, Children's Healthcare of Atlanta, Emory University School of Medicine, Emory University, Atlanta, GA, USA.
Christina CarusoDepartment of Pediatrics, Aflac Cancer Center and Blood Disorders Service, Children's Healthcare of Atlanta, Emory University School of Medicine, Emory University, Atlanta, GA, USA.ORCID 0000-0002-7453-019X
Adiya E OtumalaThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Sarah HernandezDepartment of Medicine, Division of Infectious Diseases, Emory University, Atlanta, GA, USA.
Priscilla DelgadoThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Gabrielle DeanAflac Cancer Center and Blood Disorders Service, Children's Healthcare of Atlanta, Atlanta, GA, USA.
James M KelvinThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Daniel ChesterJoint Department of Biomedical Engineering of University of North Carolina, Chapel Hill and North Carolina State University, Raleigh, NC, USA.
Ashley C BrownJoint Department of Biomedical Engineering of University of North Carolina, Chapel Hill and North Carolina State University, Raleigh, NC, USA.ORCID 0000-0001-6995-1785
Erik C DreadenThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.ORCID 0000-0002-4954-8443
Traci LeongDepartment of Biostatistics & Bioinformatics, Rollins School of Public Health, Emory University, Atlanta, GA, USA.ORCID 0000-0003-2347-4260
Jesse WaggonerJoint Department of Biomedical Engineering of University of North Carolina, Chapel Hill and North Carolina State University, Raleigh, NC, USA.
Renhao LiDepartment of Pediatrics, Aflac Cancer Center and Blood Disorders Service, Children's Healthcare of Atlanta, Emory University School of Medicine, Emory University, Atlanta, GA, USA.ORCID 0000-0002-5806-5080
Eric OrtlundDepartment of Biochemistry, Emory University School of Medicine, Emory University, Atlanta, GA, USA.ORCID 0000-0001-8855-3029
Carolyn BennettDepartment of Pediatrics, Aflac Cancer Center and Blood Disorders Service, Children's Healthcare of Atlanta, Emory University School of Medicine, Emory University, Atlanta, GA, USA.
Wilbur A LamThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. wilbur.lam@emory.edu.ORCID 0000-0002-0325-7990
David R MyersThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. david.myers@emory.edu.ORCID 0000-0001-9659-3274

Funding

Engineering biophysical microtechnologies for hematologic applications in health and diseaseR35HL145000 · NHLBI · EMORY UNIVERSITY · PI LAM, WILBUR A · 2019 to 2025
$5.0M
Leveraging platelet contraction cytometry for immune thrombocytopeniaR01HL155330 · NHLBI · EMORY UNIVERSITY · PI MYERS, DAVID RICHARD · 2021 to 2025
$1.9M
Wearable Microchip for Assessing Global HemostatsisK25HL141636 · NHLBI · EMORY UNIVERSITY · PI MYERS, DAVID RICHARD · 2019 to 2023
$788k
Novel insights on immune thrombocytopenia purpura with platelet contraction cytometryF31HL160210 · NHLBI · EMORY UNIVERSITY · PI OSHINOWO, OLUWAMAYOKUN · 2021 to 2023
$155k
Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.) F31HL160210Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.) K25HL141636Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.) R01HL155330Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.) R35HL145000NHLBI NIH HHS F31 HL160210NHLBI NIH HHS K25 HL141636NHLBI NIH HHS R01 HL155330NHLBI NIH HHS R35 HL145000
6 · The paper itself

Abstract

Altered mechanotransduction has been proposed as a putative mechanism for disease pathophysiology, yet evidence remains scarce. Here we introduce a concept we call single cell immuno-mechanical modulation, which links immunology, integrin biology, cellular mechanics, and disease pathophysiology and symptomology. Using a micropatterned hydrogel-laden coverslip compatible with standard fluorescence microscopy, we conduct a clinical mechanobiology study, specifically focusing on immune thrombocytopenia (ITP), an autoantibody-mediated platelet disorder that currently lacks a reliable biomarker for bleeding risk. We discover that in pediatric ITP patients (n = 53), low single platelet contraction force alone is a "physics-based" biomarker of bleeding (92.3% sensitivity, 90% specificity). Mechanistically, autoantibodies and monoclonal antibodies drive increases and decreases of cell force by stabilizing integrins in different conformations depending on the targeted epitope. Hence, immuno-mechanical modulation demonstrates how antibodies may pathologically alter mechanotransduction to cause clinical symptoms and this phenomenon can be leveraged to control cellular mechanics for research, diagnostic, and therapeutic purposes.

Indexed as

AutoantibodiesBlood PlateletsHemorrhageMechanotransduction, CellularAdolescentAntibodies, MonoclonalBiomarkersChildChild, PreschoolFemaleHumansIntegrinsMalePurpura, Thrombocytopenic, IdiopathicSingle-Cell AnalysisAntibodies, MonoclonalAutoantibodiesBiomarkersIntegrins

Identifiers

PMID39587073
PMCPMC11589161

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.