Evidence map›Paper›PMID 39587002›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Robust Expansion of Hematopoietic Stem Cells Ex Vivo Using Small Molecule Cocktails.

Woratree Kaewsakulthong, Ajay Ratan Pasala, Justine Hanotaux, Tanvir Hasan, Harinad B Maganti

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Woratree KaewsakulthongCanadian Blood Services, Centre for Innovation, Ottawa, ON, Canada.
Ajay Ratan PasalaCanadian Blood Services, Centre for Innovation, Ottawa, ON, Canada.
Justine HanotauxCanadian Blood Services, Centre for Innovation, Ottawa, ON, Canada.
Tanvir HasanCanadian Blood Services, Centre for Innovation, Ottawa, ON, Canada.
Harinad B MagantiCanadian Blood Services, Centre for Innovation, Ottawa, ON, Canada. harinad.maganti@blood.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The insufficient number of hematopoietic stem cells (HSCs) poses a significant challenge for successful hematopoietic stem cell transplantation and gene-based therapies. To address this issue, ex vivo expansion of HSCs has been developed, improving engraftment and reducing morbidity risks in hematological disorders. Small molecules, known as stem cell agonists (SCAs), have been utilized to promote HSC expansion and have been implemented in clinical trials. While most HSC expansion protocols focus on the single use of SCAs, we describe a protocol using an optimized small molecule cocktail (SMC), X2A, to robustly enhance HSC yield. This protocol is applicable to human CD34+ hematopoietic stem and progenitor cells (HSPCs) derived from both umbilical cord blood and peripheral blood. In addition to the ex vivo HSC expansion protocol, we detail the CD34+ HSPC isolation technique and flow cytometry methods to characterize HSPC sub-populations from cell cultures. This culture protocol serves as a robust tool for pre-clinical studies in HSPCs and provides a foundation for further modifications to meet specific research needs.

Indexed as

Cell Culture TechniquesHematopoietic Stem CellsSmall Molecule LibrariesAntigens, CD34Cell ProliferationCells, CulturedCell SeparationFetal BloodFlow CytometryHumansAntigens, CD34Small Molecule LibrariesCell expansionHematopoietic stem cellsSmall moleculesStem cell agonists

Identifiers

PMID39587002

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.