ArticleInvestigative ophthalmology & visual science2024
Metabolic Characteristics of Sclera in Lens-Induced Myopic Guinea Pigs.
Article in Investigative ophthalmology & visual science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Cross-tissue dual-omics analysis reveals molecular programs linked to myopia susceptibility and progression.Life science alliance · 2026Article
- Role of Autophagy in Scleral Remodeling During Form-Deprivation Myopia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Impaired P4HA1-Driven Hydroxyproline Formation Mediates Scleral Collagen Disruption in Form-Deprivation Myopia.Investigative ophthalmology & visual science · 2026Article
- Cellular Biological Basis and Novel Breakthroughs in the Pathogenesis of Myopia - A Narrative Review.International journal of general medicine · 2026Review
- Hypoxia-induced OPN5 expression is associated with matrix-preserving responses in human scleral fibroblasts.Frontiers in neuroscience · 2026Article
- Proteomic profiling of spontaneous myopia in guinea pigs.Frontiers in medicine · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: Myopia development is closely associated with scleral tissue loss in both human and animal models. This research aimed to investigate the metabolic changes in the sclera of lens-induced myopic guinea pigs and explore the underlying mechanisms. Methods: Myopia was induced in 2-week-old pigmented guinea pigs by having them wear -20-diopter lenses for 10 days, with one eye designated as the lens-induced myopic eye and the other as the control. Dual-platform untargeted metabolomics was performed using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) to analyze the metabolic changes in the sclera. Validation of amino acid levels in the sclera was conducted via targeted metabolomics. Glycine intervention was carried out in both scleral fibroblasts and the lens-induced myopia guinea pig model to evaluate its effects on COL1A1 synthesis and myopia progression. Results: After 10 days of lens-induced myopia, GC-MS and LC-MS analyses identified significant changes in 29 and 85 metabolites in the myopic sclera, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the downregulation of amino acid and pyrimidine metabolism pathways was crucial in myopia development. Targeted amino acid metabolomics confirmed that multiple amino acids were significantly reduced in the myopic sclera. Glycine deficiency reduced COL1A1 levels in scleral fibroblasts, and glycine supplementation significantly increased its content. Animal studies demonstrated that glycine gavage significantly inhibited axial elongation and refractive error increase in lens-induced myopic guinea pigs, increased COL1A1 content, and reversed the reduction of ferroptosis-related proteins GPX4 and FTH1. Conclusions: Several amino acids, including glycine, l-isoleucine, l-serine, and l-valine, were significantly reduced in the myopic sclera, along with a marked downregulation of amino acid and pyrimidine metabolism pathways. Glycine supplementation can increase COL1A1 content and inhibit myopia progression by reducing ferroptosis within the sclera, suggesting that glycine could serve as a potential therapeutic intervention for myopia.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.