Evidence map›Paper›PMID 39585518›Full record

ArticleJournal of assisted reproduction and genetics2025

Correlation between female pronuclear/cytoplasmic ratio and number of chromosomes in mouse zygotic stage: implications for aneuploidy assessment in ART.

Wei Xiao, Sakura Akao, Junko Otsuki

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Wei XiaoGraduate School of Environmental, Life, Natural Science and Technology, Okayama University, 1-1-1 Tsushimanaka, Kita, Okayama, 700-8530, Japan.
Sakura AkaoFaculty of Agriculture, Okayama University, 1-1-1 Tsushimanaka, Kita, Okayama, 700-8530, Japan.
Junko OtsukiGraduate School of Environmental, Life, Natural Science and Technology, Okayama University, 1-1-1 Tsushimanaka, Kita, Okayama, 700-8530, Japan. otsuki.midori.junko@gmail.com.ORCID http://orcid.org/0000-0002-8183-8651

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe objective of this study was to investigate the correlation between the pronuclear/cytoplasmic (PN/C) ratio and the number of chromosomes in mouse zygotes to understand the implications of pronuclear size regulation in early embryonic development.

methodsA combination of enucleation and aggregated chromosomes/chromatin (AC) transfer was utilized to create oocytes with varying numbers of chromosomes. Time-lapse imaging and immunofluorescence staining were employed to analyze pronuclear dynamics and chromosomal configurations.

resultsHigher chromosome numbers correspond to a larger PN/C ratio. Oocytes with a higher number of chromosomes exhibited larger pronuclei.

conclusionThe study underscores the complexity of pronuclear size regulation and its correlation with the number of chromosomes. The findings suggest potential applications in ART, where assessing the PN/C ratio could serve as a biomarker for zygote quality and aneuploidy.

Indexed as

AneuploidyCell NucleusChromosomesCytoplasmReproductive Techniques, AssistedZygoteAnimalsEmbryonic DevelopmentFemaleMiceOocytesAneuploidyChromosomeCytoplasmPN/C ratioPronucleusZygote

Identifiers

PMID39585518
PMCPMC11805731

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.