Evidence map›Paper›PMID 39585452›Full record

ArticlePlant cell reports2024

Genome-wide association study identified BnaPAP17 genes involved in exogenous ATP utilization and regulating phosphorous content in Brassica napus.

Hao Li, Haijiang Liu, Chuang Wang, Yang Zeng, Surya Kant, Xiaohua Wang, John P Hammond, Guangda Ding, Hongmei Cai, Sheliang Wang and 3 more

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Article in Plant cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

13 authors.

Hao LiNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
Haijiang LiuCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China.
Chuang WangMicroelement Research Centre, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
Yang ZengNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
Surya KantSchool of Agriculture, Biomedicine & Environment, La Trobe University, AgriBio, 5 Ring Rd, Bundoora, Vic 3083, Australia.
Xiaohua WangCollege of Agriculture and Forestry Science, Linyi University, Middle of Shuangling Road, Lanshan District, Linyi, 276000, China.
John P HammondSchool of Agriculture, Policy and Development, University of Reading, Reading, RG6 6AR, UK.
Guangda DingMicroelement Research Centre, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
Hongmei CaiMicroelement Research Centre, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
Sheliang WangMicroelement Research Centre, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
Fangsen XuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
Ying ZhangHunan Institute of Agricultural Environment and Ecology, Hunan Academy of Agricultural Sciences, Changsha, Hunan, 410125, China. zhangying@hunaas.cn.
Lei ShiNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China. leish@mail.hzau.edu.cn.ORCID http://orcid.org/0000-0002-5312-8521

Funding

Agricultural Science and Technology Innovation Funds Project of Hunan Province 2023CX105National Key Research and Development Program of China 2023YFD1700204National Natural Science Foundation of China 32172662
6 · The paper itself

Abstract

key messageBnaPAP17s associated with root-secreted APases activity were identified by genome-wide association study, and those were induced by Pi-deficiency. BnaPAP17s were involved in improving exogenous organophosphorus utilization as secreted APases. Deficiency of available phosphorus (P) in soil has become an important limiting factor for yield and quality in oilseed rape (Brassica napus). In many soils, organic P (Po) is the main component of the soil P pool. Po must be hydrolyzed to inorganic P (Pi) through acid Phosphatase (APases), and then taken up by plants. However, root-secreted APases (SAP) activity, as a quantitative trait, plays an important role in soil Po utilization; those genetic loci are not clear in B. napus. In this study, we performed a genome-wide association study for SAP activity under Pi-deficiency using a panel of 350 accessions of B. napus and more than 4.5 million polymorphic single nucleotide polymorphisms (SNPs). Thirty-five significant SNPs associated with SAP activity were identified. BnaA01.PAP17 (BnaA01g27810D) was a candidate gene underlying lead SNP (ChrA01_19576615). We experimentally verified that both BnaA01.PAP17 and its three homologous genes had similar expression pattern in response to Pi-deficiency. The dynamic changes in BnaPAP17s expression level were opposite to those of Pi concentration in both roots and leaves, suggesting their potential utility as Pi marker genes in B. napus. Transient expression of BnaPAP17s in tobacco leaves proved that BnaPAP17s were located in the apoplast as secreted APases. The overexpression of BnaPAP17s enhanced SAP activity in response to Pi-deficiency and resulting in increased P content in plants when ATP was supplied as the sole P resource. Taken together, these results suggest that BnaPAP17s contributed to SAP activity, thus having a function in extracellular Po utilization in B. napus.

Indexed as

Adenosine TriphosphateBrassica napusGenome-Wide Association StudyPhosphorusPolymorphism, Single NucleotideAcid PhosphataseGene Expression Regulation, PlantGenes, PlantPlant ProteinsPlant RootsQuantitative Trait LociAcid PhosphataseAdenosine TriphosphatePhosphorusPlant ProteinsBnaPAP17Brassica napusGenome-wide association study (GWAS)Organic phosphorus (Po)Root-secreted acid phosphatase

Identifiers

PMID39585452

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.