ArticleMethods and protocols2024
A Critical Exploration of the Total Flavonoid Content Assay for Honey.
Article in Methods and protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Physicochemical Characterization, Fatty Acid Profile, and In Vitro Antioxidant Capacity of Tumbo (Foods (Basel, Switzerland) · 2026Article
- The Effect of Temperature Variations on the 5-Hydroxymethylfurfural Content, Antioxidant and Antimicrobial Properties of Honey.Life (Basel, Switzerland) · 2026Article
- Optimisation and Validation of a Microscale FRAP Assay with Matrix-Matched (Interaction-Corrected) Blanking for Accurate Antioxidant Capacity Assessment of Honeys.Methods and protocols · 2026Article
- Physicochemical, nutritional and biological characterization of different Indian monofloral honeys with special emphasis on their glucose-lowering efficacy.Food chemistry: X · 2026Article
- Nutritional Composition, Glycemic Index, Antioxidant and Anti-Inflammatory Activities of Five Monofloral Honeys from Qassim, Saudi Arabia: A Component-Effect Relationship Study.Foods (Basel, Switzerland) · 2026Article
- Skin wound healing effects of Pectinodesmus javanensis extract through activation of TGF-β/SMAD signaling pathway.Molecular biology reports · 2026Article
- Microbiome-Metabolome Analyses Reveal Compound Risks from Multiple-Generation Cocoon Accumulation in Honeybee Combs.Biology · 2026Article
- Interaction of Red Cabbage Extract with Exogenous Antioxidants.International journal of molecular sciences · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
This study critically investigates the aluminium chloride-based colorimetric determination of the total flavonoid content (TFC) of honey. Following a comprehensive review of the recent literature reporting the use of the assay in the determination of TFC in honey, 10 honeys of different botanical origins were investigated using the colorimetric method alongside an artificial honey that was used as a control. Using spiking experiments, this study demonstrates that the flavonoid concentrations commonly found in honey are too low for a direct measurement and thus some of the TFC data reported in the literature might more likely be a reflection of the honey's inherent colour rather than a product of the coordination complex formed specifically between flavonoids and Al
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Registered trials
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