Evidence map›Paper›PMID 39583763›Full record

ArticleYonago acta medica2024

An Optimal Transport Medium for SARS-CoV-2 Detection in the Direct Method of Rapid Microfluidic PCR System.

Miyako Takata, Masaki Nakamoto, Tsuyoshi Kitaura, Kensaku Okada, Hiroko Endou, Athok Shofiudin Ma'arif, Yukari Nishikawa, Kengo Mukuda, Shota Morishita, Hiromi Murota and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Yonago acta medica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Miyako TakataDepartment of Pathobiological Science and Technology, Graduate School of Medical Science, School of Health Science, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan.
Masaki NakamotoDivision of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori university, Yonago 683-8503, Japan.
Tsuyoshi KitauraDivision of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori university, Yonago 683-8503, Japan.
Kensaku OkadaDivision of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori university, Yonago 683-8503, Japan.
Hiroko EndouWatarase Research Center Kyorin Pharmaceutical Co., Ltd., Shimotsuga 329-0114, Japan.
Athok Shofiudin Ma'arifDivision of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori university, Yonago 683-8503, Japan.
Yukari NishikawaDivision of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori university, Yonago 683-8503, Japan.
Kengo MukudaDivision of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori university, Yonago 683-8503, Japan.
Shota MorishitaDivision of Clinical Laboratory, Tottori University Hospital, Yonago 683-8504, Japan.
Hiromi MurotaDivision of Clinical Laboratory, Tottori University Hospital, Yonago 683-8504, Japan.
Akira YamasakiDivision of Respiratory medicine and Rheumatology, Graduate School of Medical Sciences, School of Medicine, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan.
Seiji KageyamaDivision of virology, Department of Microbiology and Immunology, School of Medicine, Faculty of Medicine, Tottori university, Yonago 683-8503, Japan.
Naoto BuriokaDepartment of Pathobiological Science and Technology, Graduate School of Medical Science, School of Health Science, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan.
Hiroki ChikumiDivision of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori university, Yonago 683-8503, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Recently developed rapid real-time reverse transcription PCR (RT-PCR) systems adopting microfluidic thermal cycling technology are ideal for point-of-care (POC) testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Because the RNA extraction step before real-time RT-PCR is rate-limiting, a direct RNA extraction method (direct method) that adopts chemical viral lysis and eliminates RNA purification steps is preferable for rapid real-time RT-PCR. In the direct method, selecting the transport medium is essential because it may be introduced into subsequent real-time RT-PCR steps, but might inhibit PCR. However, the influence of transport medium on the combination of the direct method and rapid real-time RT-PCR has been yet unstudied. In the present study, we examined the influence of various transport mediums when combining the direct method and rapid real-time RT-PCR of GeneSoC Methods: To explore the influence of the transport medium on the GeneSoC Results: While only 1 copy/reaction of RNA was detected in H Conclusion: The combination of the direct method and microfluidic rapid PCR machine GeneSoC

Indexed as

direct methodGeneSoC®rapid PCRSARS-CoV-2transport medium

Identifiers

PMID39583763
PMCPMC11584235

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.