In-depth exploration of microbial electrolysis cell coupled with anaerobic digestion (MEC-AD) for methanogenesis in treating protein wastewater at high organic loading rates.

High concentrations of protein wastewater often reduce treatment efficiency due to ammonia inhibition and acid accumulation caused by its low carbon-to-nitrogen ratio (C/N) after digestion, as well as its complex structure. This study investigates the performance of a microbial electrolysis cell (MEC) driving a protein digestion system with gradually increasing organic loading rates (OLR) of bovine serum albumin, elucidating microbial changes and methanogenic metabolic pathways on bioelectrodes under high OLR "inhibited steady-state" (ISS) conditions. The results showed that the accumulation of ammonia nitrogen (AN) from protein hydrolysis under high OLR conditions disrupted microbial growth and caused cell death on the electrode surface, hindering the electron transfer rate. Toxic AN reduced protein hydrolysis, led to propionate accumulation, inhibiting the acetoclastic methanogenesis process and favoring the hydrogenotrophic pathway. As OLR increased from 6 to 11 gCOD/L, cumulative methane production increased significantly from 450.24 mL to 738.72 mL, while average methane yield and production rate decreased by 10.51% and 50.28%, from 375.20 mL/gCOD and 75.04 mL/(gCOD·d) to 335.78 mL/gCOD and 37.31 mL/(gCOD·d), respectively. Despite these declines, the system maintained an ISS. Moderate OLR increases can achieve an ISS, boosting protein waste treatment capacity, methane production, and net energy output (NEO), with an OLR of 6 gCOD/L being optimal for maximizing NEO per unit substrate. These findings provide theoretical insights into the methanogenesis pathway of high OLR proteins in MEC-AD systems and offer an effective method for treating high OLR protein wastewater in future practical applications.