Evidence map›Paper›PMID 39581586›Full record

ArticleThe Journal of veterinary medical science2025

Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines.

Mami Oba, Ryo Imai, Natsuko Teshima, Miho Noda, Yuka Nunomura, Seiichi Kakinuma, Kazuki Ito, Masashi Oguro, Kazunari Konaka, Tomoya Enomoto and 4 more

Abstract read
In one paragraph

Article in The Journal of veterinary medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Mami ObaCenter for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Ryo ImaiCenter for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Natsuko TeshimaCenter for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Miho NodaCenter for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Yuka NunomuraCenter for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Seiichi KakinumaKakinuma Veterinary Hospital, Saitama, Japan.
Kazuki ItoVeterinary Clinic, Saitama Agricultural Mutual Aid Association, Saitama, Japan.
Masashi OguroMYDS Corporation, Gunma, Japan.
Kazunari KonakaKM Clinic, Tochigi, Japan.
Tomoya EnomotoVeterinary Clinic, Kanagawa Agricultural Mutual Aid Association, Kanagawa, Japan.
Hisamoto MoritaMorita Veterinary Hospital, Kanagawa, Japan.
Tsutomu OmatsuCenter for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Makoto NagaiSchool of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Tetsuya MizutaniCenter for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo-PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo-PCR may be useful in elucidating new pathologies associated with multiple pathogens.

Indexed as

Cattle DiseasesQuarantineReal-Time Polymerase Chain ReactionAnimalsCattleJapancattlecomprehensiveDembo-PCRpathogenreal-time PCR

Identifiers

PMID39581586
PMCPMC11735209

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.