ArticleThe Journal of cell biology1986
Role of stress fiber-like structures in assembling nascent myofibrils in myosheets recovering from exposure to ethyl methanesulfonate.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 81 citations in OpenAlex.
- Review
- Gastrocnemius Medialis Architectural Properties in Flexibility Trained and Not Trained Child Female Athletes: A Pilot Study.Sports (Basel, Switzerland) · 2020Article
- Activity of cofilin can be regulated by a mechanism other than phosphorylation/dephosphorylation in muscle cells in culture.Journal of muscle research and cell motility · 2007Article
- Assembly of transverse tubule architecture in the middle and myotendinous junctional regions in developing rat skeletal muscle fibers.Journal of muscle research and cell motility · 2007Article
- Cardiomyocyte cytoskeleton and myofibrillogenesis in healthy and diseased heart.Heart failure reviews · 2000Review
- Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin.The Journal of cell biology · 2000Article
- Remodelling of adult cardiac muscle cells in culture: dynamic process of disorganization and reorganization of myofibrils.Journal of muscle research and cell motility · 1996Article
- Intermediate filament proteins in TPA-treated skeletal muscle cells in culture.Journal of muscle research and cell motility · 1996Article
- Heparin and the phenotype of adult human vascular smooth muscle cells.In vitro cellular & developmental biology. Animal · 1995Article
- Involvement of transglutaminase in myofibril assembly of chick embryonic myoblasts in culture.The Journal of cell biology · 1995Article
- Mechanisms of thin filament assembly in embryonic chick cardiac myocytes: tropomodulin requires tropomyosin for assembly.The Journal of cell biology · 1995Article
- Assembly of body wall muscle and muscle cell attachment structures in Caenorhabditis elegans.The Journal of cell biology · 1994Article
- Segregated assembly of muscle myosin expressed in nonmuscle cells.Molecular biology of the cell · 1993Article
- Remodelling of cardiomyocyte cytoarchitecture visualized by three-dimensional (3D) confocal microscopy.Histochemistry · 1993Article
- Incorporation of microinjected biotin-labelled actin into nascent myofibrils of cardiac myocytes: an immunoelectron microscopic study.Journal of muscle research and cell motility · 1993Article
- The vinculin/sarcomeric-alpha-actinin/alpha-actin nexus in cultured cardiac myocytes.The Journal of cell biology · 1992Article
- Vinculin is essential for muscle function in the nematode.The Journal of cell biology · 1991Article
- Chicken cardiac myofibrillogenesis studied with antibodies specific for titin and the muscle and nonmuscle isoforms of actin and tropomyosin.Cell and tissue research · 1991Article
- Myosin mRNA accumulation and myofibrillogenesis at the myotendinous junction of stretched muscle fibers.The Journal of cell biology · 1990Article
- Assembly of connectin (titin) in relation to myosin and alpha-actinin in cultured cardiac myocytes.Journal of muscle research and cell motility · 1990Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
When day 1 cultures of chick myogenic cells were exposed to the mutagenic alkylating agent ethyl methanesulfonate (EMS) for 3 d, 80% of the replicating cells were killed, but postmitotic myoblasts survived. The myoblasts fused to form unusual multinucleated "myosheets": extraordinarily wide, flattened structures that were devoid of myofibrils but displayed extensive, submembranous stress fiber-like structures (SFLS). Immunoblots of the myosheets indicated that the carcinogen blocked the synthesis and accumulation of the myofibrillar myosin isoforms but not that of the cytoplasmic myosin isoform. When removed from EMS, widely spaced nascent myofibrils gradually emerged in the myosheets after 3 d. Striking co-localization of fluorescent reagents that stained SFLS and those that specifically stained myofibrils was observed for the next 2 d. By both immunofluorescence and electron microscopy, individual nascent myofibrils appeared to be part of, or juxtaposed to, preexisting individual SFLS. By day 6, all SFLS had disappeared, and the definitive myofibrils were displaced from their submembranous site into the interior of the myosheet. Immunoblots from recovering myosheets demonstrated a temporal correlation between the appearance of the myofibrillar myosin isoforms and the assembly of thick filaments. The assembly of definitive myofibrils did not appear to involve desmin intermediate filaments, but a striking aggregation of sarcoplasmic reticulum elements was seen at the level of each I-Z-band. Our findings suggest that SFLS in the EMS myosheets function as early, transitory assembly sites for nascent myofibrils.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.