ArticleThe Journal of cell biology1986
Actin at receptor-rich domains of isolated acetylcholine receptor clusters.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 76 citations in OpenAlex.
- Alteration of actin cytoskeletal organisation in fetal akinesia deformation sequence.Scientific reports · 2024Article
- Myosin Va: Capturing cAMP for synaptic plasticity.Frontiers in physiology · 2023Review
- To build a synapse: signaling pathways in neuromuscular junction assembly.Development (Cambridge, England) · 2010Review
- Beta-catenin regulates acetylcholine receptor clustering in muscle cells through interaction with rapsyn.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- The role of the cytoskeleton in neuromuscular junction formation.Journal of molecular neuroscience : MN · 2006Article
- The actin-driven movement and formation of acetylcholine receptor clusters.The Journal of cell biology · 2000Article
- Disruption of actin impedes transmitter release in snake motor terminals.The Journal of physiology · 2000Article
- Cytoplasmic Orientation of the Naphthylphthalamic Acid-Binding Protein in Zucchini Plasma Membrane Vesicles.Plant physiology · 1996Article
- The desensitization of the embryonic mouse muscle acetylcholine receptor depends on the cellular environment.Pflugers Archiv : European journal of physiology · 1995Article
- NPA binding activity is peripheral to the plasma membrane and is associated with the cytoskeleton.The Plant cell · 1994Article
- Association of acetylcholine receptors with peripheral membrane proteins: evidence from antibody-induced coaggregation.The Journal of membrane biology · 1994Article
- Clustering and immobilization of acetylcholine receptors by the 43-kD protein: a possible role for dystrophin-related protein.The Journal of cell biology · 1993Article
- Tyrosine phosphorylation and acetylcholine receptor cluster formation in cultured Xenopus muscle cells.The Journal of cell biology · 1993Article
- Clusters of neural cell adhesion molecule at sites of cell-cell contact.The Journal of cell biology · 1992Article
- Nicotinic receptor-associated 43K protein and progressive stabilization of the postsynaptic membrane.Molecular neurobiology · 1992Review
- Cytoplasmic components of acetylcholine receptor clusters of cultured rat myotubes: the 58-kD protein.The Journal of cell biology · 1991Article
- The submembrane machinery for nicotinic acetylcholine receptor clustering.The Journal of cell biology · 1991Review
- A protein homologous to the Torpedo postsynaptic 58K protein is present at the myotendinous junction.The Journal of cell biology · 1990Article
- Rotational diffusion of acetylcholine receptors on cultured rat myotubes.The Journal of cell biology · 1990Article
- Microinjection of a monoclonal antibody against a 37-kD protein (tropomyosin 2) prevents the formation of new acetylcholine receptor clusters.The Journal of cell biology · 1989Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
Acetylcholine receptor (AChR) clusters of cultured rat myotubes, isolated by extraction with saponin (Bloch, R. J., 1984, J. Cell Biol. 99:984-993), contain a polypeptide that co-electrophoreses with purified muscle actins. A monoclonal antibody against actin reacts in immunoblots with this polypeptide and with purified actins. In indirect immunofluorescence, the antibody stains isolated AChR clusters only at AChR domains, strips of membrane within clusters that are rich in receptor. It also stains the postsynaptic region of the neuromuscular junction of adult rat skeletal muscle. Semiquantitative immunofluorescence analyses show that labeling by antiactin of isolated analyses show that labeling by antiactin of isolated AChR clusters is specific and saturable and that it varies linearly with the amount of AChR in the cluster. Filaments of purified gizzard myosin also bind preferentially at AChR-rich regions, and this binding is inhibited by MgATP. These experiments suggest that actin is associated with AChR-rich regions of receptor clusters. Depletion of actin by extraction of isolated clusters at low ionic strength selectively releases the actin-like polypeptide from the preparation. Simultaneously, AChRs redistribute within the plane of the membrane of the isolated clusters. Similarly, brief digestion with chymotrypsin reduces immunofluorescence staining and causes AChR redistribution. Treatments that deplete AChR from clusters in intact cells also reduce immunofluorescent staining for actin in isolated muscle membrane fragments. Upon reversal of these treatments, cluster reformation occurs in regions of the membrane that also stain for actin. I conclude that actin is associated with AChR domains and that changes in this association are accompanied by changes in the organization of isolated AChR clusters.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.