Evidence map›Paper›PMID 39580521›Full record

ArticleScientific reports2024

Expression of variant isoforms of the tyrosine kinase SYK differentially regulates cervical cancer progression through PI3K/AKT pathway.

Bingjie Lin, Qixin Wang, Xin Wang, Hongjian Wei, Xiaojing Nie, Li Li, Yonghua Shi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Bingjie Lin *Department of Pathology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang, 830017, China.
Qixin Wang *Department of Pathology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang, 830017, China.
Xin WangDepartment of Pathology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang, 830017, China.
Hongjian WeiDepartment of Pathology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang, 830017, China.
Xiaojing NieDepartment of Pathology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang, 830017, China.
Li LiDepartment of Gynecology, Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China. donghui555@sina.com.
Yonghua ShiDepartment of Pathology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang, 830017, China. shiyonghua@xjmu.edu.cn.

Funding

National Natural Science Foundation of China 81660427Natural Science Foundation Project of Xinjiang Autonomous Region 2021D01A47
6 · The paper itself

Abstract

Invasion and metastasis are the main reasons for the poor prognosis of patients with cervical cancer(CC). SYK is closely related to tumor development. However, the functions of its two isoforms, SYK (L) or SYK (S), are not fully understood to date. In this study, we investigated their biologic functions and possible prognostic values in CC. qRT-PCR was performed to detect the expression of SYK and two variant isoforms in cervical cancer tissues and cells. The association of SYK(L) and SYK(S) with Clinical pathological parameters were evaluated. The migration and invasion was detected by scratch assay and transwell. Western blot was conducted to measure the changes of epithelial mesenchymal transition (EMT)-related markers and PI3K/AKT signaling pathway proteins in cervical cancer cells. LY294002 (inhibitor of PI3K/AKT pathway) and IGF-1 (activator of PI3K/AKT pathway) were applied to evaluate the contribution of PI3K/AKT signaling pathway in cervical cancer cells. The expression of SYK(S) in cervical cancer tissues was significantly higher than that of SYK(L). SYK(L) and SYK(S) were correlated with muscular infiltration, SYK(L) high expression had a better prognosis, whereas SYK(S) high expression predicted a worse disease outcome. Cox multivariate regression analysis demonstrated that SYK(L) expression was an independent prognostic factor. SYK(L) significantly inhibited the proliferation, migration and invasion, while SYK(S) showed the opposite effects. LY294002 blocked SYK (L) knockdown-induced enhancement of migration and invasion as well as the expression EMT-related markers, whereas IGF-1 rescued the decreased migration, invasion and EMT induced by SYK (S) knockdown. The results suggest that SYK(L) and SYK(S) are involved in the progression of cervical cancer through PI3K/AKT signaling pathway, and may serve as potential targets for clinical treatment of advanced cervical cancer.

Indexed as

Cell MovementDisease ProgressionPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionSyk KinaseUterine Cervical NeoplasmsAdultCell Line, TumorChromonesEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticHumansMiddle AgedMorpholinesChromonesMorpholinesPhosphatidylinositol 3-KinasesProtein IsoformsProto-Oncogene Proteins c-aktSyk KinaseSYK protein, humanCervical cancerPI3K/AKTPrognosisSYK(L)SYK(S)

Identifiers

PMID39580521
PMCPMC11585633

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.