Evidence map›Paper›PMID 39579355›Full record

ArticleCell reports2024

Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferation.

Qin Ni, Zhuoxu Ge, Yizeng Li, Gabriel Shatkin, Jinyu Fu, Anindya Sen, Kaustav Bera, Yuhan Yang, Yichen Wang, Yufei Wu and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Review
  2. Cell-nanoplastics association impacts cell proliferation and motility.bioRxiv : the preprint server for biology · 2026
    Article
  3. Acute priming using elevated fluid viscosity recovers 'bioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Chemical compensation to mechanical loss in cell mechanosensation.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  6. Article
  7. Article
  8. Pump up the volume.eLife · 2024
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Qin NiInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Zhuoxu GeInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Yizeng LiDepartment of Biomedical Engineering, Binghamton University, Binghamton, NY, USA.
Gabriel ShatkinDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Jinyu FuInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Physics, Johns Hopkins University, Baltimore, MD, USA.
Anindya SenInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Kaustav BeraInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Yuhan YangDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Yichen WangDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Yufei WuInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Ana Carina Nogueira VasconcelosInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Yuqing YanInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA.
Dingchang LinInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA.
Andrew P FeinbergDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA; Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Center for Epigenetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Konstantinos KonstantopoulosInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA; Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Sean X SunInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA. Electronic address: ssun@jhu.edu.

Funding

The interplay of ion transporters and cytoskeleton in breast cancer migration and metastasisR01CA254193 · NCI · JOHNS HOPKINS UNIVERSITY · PI KONSTANTOPOULOS, KONSTANTINOS, MARTIN, STUART S · 2021 to 2025
$2.7M
The Role of Hydraulic Pressure in the Osmotic Engine Model of Cell MigrationR01GM134542 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI SEAN X SUN · 2019 to 2026
$2.1M
Protein assemblies as genetically encoded mechanical actuators for intracellular mechanobiology researchR35GM147274 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Dingchang Lin · 2022 to 2026
$2.0M
Cell mechanobiology in confinement using an integration of bioengineering, materials systems and in vivo modelsR01GM142175 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI KONSTANTOPOULOS, KONSTANTINOS · 2021 to 2024
$1.8M
NCI NIH HHS R01 CA254193NIGMS NIH HHS R01 GM134542NIGMS NIH HHS R01 GM142175NIGMS NIH HHS R35 GM147274
6 · The paper itself

Abstract

Mammalian cells rapidly respond to environmental changes by altering transmembrane water and ion fluxes, changing cell volume. Contractile forces generated by actomyosin have been proposed to mechanically regulate cell volume. However, our findings reveal a different mechanism in adherent cells, where elevated actomyosin activity increases cell volume in normal-like cells (NIH 3T3 and others) through interaction with the sodium-hydrogen exchanger isoform 1 (NHE1). This leads to a slow secondary volume increase (SVI) following the initial regulatory volume decrease during hypotonic shock. The active cell response is further confirmed by intracellular alkalinization during mechanical stretch. Moreover, cytoskeletal activation of NHE1 during SVI deforms the nucleus, causing immediate transcriptomic changes and ERK-dependent growth inhibition. Notably, SVI and its associated changes are absent in many cancer cell lines or cells on compliant substrates with reduced actomyosin activity. Thus, actomyosin acts as a sensory element rather than a force generator during adaptation to environmental challenges.

Indexed as

ActomyosinCell ProliferationCell SizeCytoskeletonSodium-Hydrogen Exchanger 1Adaptation, PhysiologicalAnimalsHumansMechanotransduction, CellularMiceNIH 3T3 CellsActomyosinSlc9a1 protein, mouseSodium-Hydrogen Exchanger 1cell volumeCP: Cell biologycytoskeletonepigenomeERK/MAPKmath modelmechanosensationNHE1nucleus volumePI3KRNA-seq

Identifiers

PMID39579355
PMCPMC11871582

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.