Evidence map›Paper›PMID 39579322›Full record

ArticleApplied biochemistry and biotechnology2025

Tripartite Motif Containing 71 Suppresses Tumor Growth by Down-Regulating eIF5A2 Expression in Laryngeal Squamous Cell Carcinoma.

Dan Lou, Jianxing Wang, Haizhong Zhang, Qiaojing Jia, Lisha Liu, Yanrui Bian, Yue Di, Chunguang Shan

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Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Dan LouDepartment of Otolaryngology, the Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Jianxing WangDepartment of Otolaryngology, the Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Haizhong ZhangDepartment of Otolaryngology, the Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Qiaojing JiaDepartment of Otolaryngology, the Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Lisha LiuDepartment of Otolaryngology, the Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Yanrui BianDepartment of Otolaryngology, the Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Yue DiDepartment of Otolaryngology, the First Hospital of Qinhuangdao, Qinhuangdao, 066000, Hebei, China.
Chunguang ShanDepartment of Otolaryngology, the Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China. shanchunguang0825@hb2h.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The incidence of laryngeal squamous cell carcinoma (LSCC) has been rising recently. LSCC is one of the most prevalent malignant tumors of the head and neck. In this study, we aimed to investigate whether tripartite motif containing 71 (TRIM71) could serve as a molecular target for the treatment of LSCC. The mRNA and protein levels were examined by using real-time qPCR and Western blot, respectively. Cell proliferation was determined by cell-counting kit 8 assay. To further confirm the function of TRIM71 in LSCC, an in vivo cell line-derived xenograft study was conducted. The half-life of eukaryotic translation initiation factor 5A2 (eIF5A2) protein was measured by cycloheximide chase assay. Our results showed that TRIM71 was significantly downregulated in LSCC tumor tissues. TRIM71 overexpression significantly inhibited LSCC cell growth and suppressed tumor volume and weight in the xenograft models. The interaction between TRIM71 and eIF5A2 was verified by co-immunoprecipitation assay. Moreover, overexpression of TRIM71 in LSCC cells significantly inhibited the protein expression of eIF5A2 by down-regulating its stability, while it did not affect its mRNA level. In contrast, overexpression of eIF5A2 abolished the anti-tumor effects of TRIM71. In summary, TRIM71 may exert its anti-tumor effects through regulating eIF5A2, highlighting the potential of TRIM71 as an effective therapeutic target for the treatment of LSCC.

Indexed as

Carcinoma, Squamous CellDown-RegulationGene Expression Regulation, NeoplasticLaryngeal NeoplasmsNeoplasm ProteinsPeptide Initiation FactorsRNA-Binding ProteinsTripartite Motif ProteinsAnimalsCell Line, TumorCell ProliferationEukaryotic Translation Initiation Factor 5AHumansMaleMiceMice, Inbred BALB CEukaryotic Translation Initiation Factor 5ANeoplasm ProteinsPeptide Initiation FactorsRNA-Binding ProteinsTripartite Motif ProteinsEIF5A2Laryngeal squamous cell carcinomaProtein stabilityTRIM71

Identifiers

PMID39579322

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.