ArticleMethods in molecular biology (Clifton, N.J.)2025
Analysis of the Pattern of RNA Expression in the Skin of TR-Deficient Mice By RNA-seq.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
RNA-sequencing (RNA-seq) has become the method of choice for whole transcriptome analysis, as it enables profiling of a wide range of RNA molecules and the always evolving bioinformatic pipelines allow the extraction of diverse biological information from a single sample. However, there is not a gold-standard RNA-seq protocol nor an optimal bioinformatic pipeline defined, which can challenge new users. The aim of this chapter is to describe the basic RNA-sequencing pipeline, from RNA extraction and library preparation to the bioinformatic preprocessing and downstream analyses. The steps are oriented to the transcriptome analysis of the skin of TR-deficient mice, but the protocol can be easily translated to other tissue types or organisms.
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