Evidence map›Paper›PMID 39578323›Full record

ArticleAnnals of surgical oncology2025

Intratumoral Bacteria are Uncommon in Gastrointestinal Stromal Tumor.

Katherine J Tardy, Hyunjee V Kwak, Andrew D Tieniber, Alina K Mangold, Juan E Perez, Kevin Do, Shan Zeng, Ferdinando Rossi, Ronald P DeMatteo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of surgical oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Katherine J Tardy *Department of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Hyunjee V Kwak *Department of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Andrew D TieniberDepartment of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Alina K MangoldDepartment of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Juan E PerezDepartment of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Kevin DoDepartment of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Shan ZengDepartment of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Ferdinando RossiDepartment of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Ronald P DeMatteoDepartment of Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. dematter@upenn.edu.

Funding

Molecular Mechanisms in Gastrointestinal Stromal TumorR01CA102613 · NCI · UNIVERSITY OF PENNSYLVANIA · PI Ronald P. Dematteo · 2004 to 2026
$9.2M
SURGICAL ONCOLOGY RESEARCH TRAINING PROGRAM AT PENNT32CA251063 · NCI · UNIVERSITY OF PENNSYLVANIA · PI Ronald P. Dematteo · 2020 to 2026
$2.6M
Basic Research Laboratory R01 CA102613-18Basic Research Laboratory T32 CA251063-04NCI NIH HHS R01 CA102613NCI NIH HHS T32 CA251063
6 · The paper itself

Abstract

backgroundGastrointestinal stromal tumor (GIST) is the most common human sarcoma with over 5000 new patients diagnosed in the USA each year. The tumor originates from the interstitial cells of Cajal and forms an intramural lesion most commonly in the stomach or small intestine. The gut microbiome has been linked to other gastrointestinal cancers and a recent paper purported that GISTs contain substantial intratumoral bacteria. The purpose of this study is to further evaluate the presence of bacteria in GISTs. PATIENTS AND

methodsWe collected 25 tumor samples of varying size and location from 24 patients under sterile conditions in the operating room immediately following surgical resection. 16S quantitative polymerase chain reaction (qPCR) and 16S ribosomal RNA (rRNA) gene amplicon sequencing were performed to evaluate the bacterial species present in each tumor. Retrospective chart review was performed to determine tumor characteristics, including tumor size, location, imatinib exposure, and mucosal involvement.

resultsIn 23 of the 25 tumor samples, there were fewer than 100 copy numbers of 16S rRNA per uL, indicating an absence of a significant bacterial load. 16S rRNA gene amplicon sequencing of the remaining two samples, one gastric tumor and one duodenal tumor, revealed the presence of normal intestinal bacteria. These two tumors, along with three others, had disruption of the mucosal lining.

conclusionsGISTs generally lack substantial bacteria, except in some cases when the tumor disrupts the mucosa.

Indexed as

BacteriaGastrointestinal MicrobiomeGastrointestinal NeoplasmsGastrointestinal Stromal TumorsAdultAgedAged, 80 and overFemaleFollow-Up StudiesHumansMaleMiddle AgedPrognosisRetrospective StudiesRNA, Ribosomal, 16SRNA, Ribosomal, 16S

Identifiers

PMID39578323
PMCPMC11811456

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.