Evidence map›Paper›PMID 39576606›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Characterizations of Nuclear Organization and Chromatin Modifications in Mature Embryos from Arabidopsis Dry Seeds.

Lauriane Simon, Manon Verdier, Aline V Probst

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lauriane SimoniGReD, CNRS, Inserm, Université Clermont Auvergne, Clermont-Ferrand, France.
Manon VerdieriGReD, CNRS, Inserm, Université Clermont Auvergne, Clermont-Ferrand, France.
Aline V ProbstiGReD, CNRS, Inserm, Université Clermont Auvergne, Clermont-Ferrand, France. aline.probst@uca.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Embryo development and seed germination are characterized by important alterations in nuclear and chromatin organization. It is however challenging to analyze these dynamic changes because the Arabidopsis embryo is embedded in the seed coat and a layer of endosperm tissue and is therefore difficult to access. Here, we describe a protocol to analyze nuclear organization in whole-mount dry seed embryos from Arabidopsis thaliana and to investigate the distribution of histone variants and modifications using immunostaining on isolated embryo nuclei. This protocol should be useful to elucidate the dry seed-specific chromatin organization and to follow dynamic changes in nuclear organization during seed germination.

Indexed as

ArabidopsisCell NucleusChromatinHistonesSeedsArabidopsis ProteinsEndospermGerminationArabidopsis ProteinsChromatinHistonesArabidopsis thaliana embryoHistone modificationsImmunostainingNuclear organizationSeed

Identifiers

PMID39576606

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.