ArticleDevelopment (Cambridge, England)2024
A new cell culture resource for investigations of reptilian gene function.
Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Isolating and Validating Fibroblast-like Cells from the Skeletal Muscle of the Siamese Crocodile (Veterinary sciences · 2026Article
- Article
- Pre-oviposition development of the brown anole (Anolis sagrei).Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Oogenesis and germinal bed morphology of the brown anole (A. sagrei).Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Oogenesis and germinal bed morphology of the brown anole (bioRxiv : the preprint server for biology · 2025Article
- Selection and evaluation of dual reference genes in silkwormScience progressArticle
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Authors and funding
7 authors.
Funding
Abstract
The establishment of CRISPR/Cas9 gene editing in Anolis sagrei has positioned this species as a powerful model for studies of reptilian gene function. To enhance this model, we developed an immortalized lizard fibroblast cell line (ASEC-1) for the exploration of reptilian gene function in cellular processes. We demonstrate the use of this cell line by scrutinizing the role of primary cilia in lizard Hedgehog (Hh) signaling. Using CRISPR/Cas9 mutagenesis, we disrupted the ift88 gene, which is required for ciliogenesis in diverse organisms. We determined that loss of itf88 from lizard cells leads to an absence of primary cilia, a partial derepression of gli1 transcription, and an inability of the cells to respond to the Smoothened agonist, SAG. Through a cross-species analysis of SAG-induced transcriptional responses in cultured limb bud cells, we further determined that ∼46% of genes induced as a response to Hh pathway activation in A. sagrei are also SAG responsive in Mus musculus limb bud cells. Our results highlight conserved and diverged aspects of Hh signaling in anoles and establish a new resource for investigations of reptilian gene function.
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Registered trials
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