Evidence map›Paper›PMID 39575928›Full record

ArticleDevelopment (Cambridge, England)2024

Cellular retinoic acid-binding proteins regulate germ cell proliferation and sex determination in zebrafish.

Lianna Fung, Daniel B Dranow, Arul Subramanian, Natalia Libby, Thomas F Schilling

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Regulation of early gonocyte differentiation in zebrafish.Biochemical Society transactions · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lianna FungDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.ORCID 0000-0002-4322-6988
Daniel B DranowDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.ORCID 0000-0002-5685-3833
Arul SubramanianDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.ORCID 0000-0001-8455-6804
Natalia LibbyDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.
Thomas F SchillingDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.ORCID 0000-0003-1798-8695

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
Uncover Strategies Used By Biological SystemsP50GM076516 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NIE, QING · 2007 to 2016
$25.1M
THE ROLE OF ATROPHIN-2 IN CARTILAGE PATTERNING AND POLARITY IN ZEBRAFISHR01DE013828 · NIDCR · UNIVERSITY OF CALIFORNIA-IRVINE · PI SCHILLING, THOMAS F · 2001 to 2025
$9.1M
Regulation of Morphogenesis and Extracellular Matrix Assembly at the Myotendinous JunctionR01AR067797 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Thomas F Schilling · 2016 to 2026
$3.5M
Dissecting single cell dynamics that coordinate neural crest migration and diversificationR01DE030565 · NIDCR · UNIVERSITY OF CALIFORNIA-IRVINE · PI NIE, QING, SCHILLING, THOMAS F · 2021 to 2025
$2.8M
National Science Foundation MCB2028424NCI NIH HHS P30 CA062203NIAMS NIH HHS R01 AR067797NIDCR NIH HHS R01 DE013828NIDCR NIH HHS R01 DE030565NIGMS NIH HHS P50 GM076516NIH HHS R01 DE13828NIH HHS RO1DE30565Simons Foundation 594598University of California
6 · The paper itself

Abstract

Cellular retinoic acid (RA)-binding proteins (Crabps) solubilize intracellular RA and transport it to its nuclear receptors or cytoplasmic degradation enzymes. Despite their extreme conservation across chordates, genetic studies of Crabp function have revealed few essential functions. We have generated loss-of-function mutations in all four zebrafish Crabps and find essential roles for Crabp2 proteins in gonad development and sex determination. Transgenic RA reporters show strong RA responses in germ cells at the bipotential stage of gonad development. Double mutants lacking the functions of both Crabp2a and Crabp2b predominantly become male, which correlates with their smaller gonad size and reduced germ cell proliferation during gonad development at late larval and early juvenile stages. In contrast, mutants lacking the functions of both Crabp1a and Crabp1b have normal sex ratios. Exogenous RA treatments at bipotential gonad stages increase germ cell number, consistent with a direct role for RA in promoting germ cell proliferation. Our results suggest essential functions for Crabps in gonad development and sex determination.

Indexed as

Cell ProliferationGerm CellsGonadsReceptors, Retinoic AcidSex Determination ProcessesTretinoinZebrafishZebrafish ProteinsAnimalsAnimals, Genetically ModifiedFemaleGene Expression Regulation, DevelopmentalMaleMutationReceptors, Retinoic Acidretinoic acid binding protein I, cellularretinoic acid binding protein II, cellularTretinoinZebrafish ProteinsCrabpGerm cellGonad developmentRetinoic acidSex determinationSex differentiation

Identifiers

PMID39575928
PMCPMC11664166

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.