Evidence map›Paper›PMID 39574693›Full record

ArticlebioRxiv : the preprint server for biology2024

Nascent transcript O-MAP reveals the molecular architecture of a single-locus subnuclear compartment built by RBM20 and the

Evan E Kania, Aidan Fenix, Daphnée M Marciniak, Qiaoyi Lin, Sara Bianchi, Borislav Hristov, Shuai Li, Conor K Camplisson, Rose Fields, Brian J Beliveau and 6 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Evan E KaniaDepartment of Pharmacology, University of Washington, Seattle, WA, USA.ORCID 0000-0002-0266-6610
Aidan FenixInstitute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.ORCID 0000-0002-1564-7347
Daphnée M MarciniakDepartment of Pharmacology, University of Washington, Seattle, WA, USA.
Qiaoyi LinDepartment of Pharmacology, University of Washington, Seattle, WA, USA.
Sara BianchiMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.
Borislav HristovDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0000-0002-1414-8378
Shuai LiDepartment of Pharmacology, University of Washington, Seattle, WA, USA.
Conor K CamplissonDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0000-0001-5085-7924
Rose FieldsDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0009-0006-3365-4598
Brian J BeliveauInstitute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.ORCID 0000-0003-1314-3118
Devin K SchweppeDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0000-0002-3241-6276
William S NobleDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0000-0001-7283-4715
Shao-En OngDepartment of Pharmacology, University of Washington, Seattle, WA, USA.ORCID 0000-0003-3314-5903
Alessandro BerteroMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.ORCID 0000-0002-4919-9087
Charles E MurryInstitute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.ORCID 0000-0003-3862-6773
David M ShechnerDepartment of Pharmacology, University of Washington, Seattle, WA, USA.ORCID 0000-0002-0574-256X

Funding

Pharmacological Sciences SupplementT32GM007750 · NIGMS · UNIVERSITY OF WASHINGTON · PI ATKINS, WILLIAM M · 1985 to 2023
$12.6M
Probing the dynamics of chromosome organization in single cellsR35GM137916 · NIGMS · UNIVERSITY OF WASHINGTON · PI Brian Joseph Beliveau · 2020 to 2026
$2.9M
Function, composition, and mechanism of RNA splicing factories in cardiomyopathyR01HL160825 · NHLBI · UNIVERSITY OF WASHINGTON · PI Charles E Murry · 2023 to 2026
$2.3M
Oligonucleotide-directed in situ proximity biotinylation: a unified method for mapping RNA-interacting proteomes, transcriptomes and genomic loci within intact cells.R01GM138799 · NIGMS · UNIVERSITY OF WASHINGTON · PI SHECHNER, DAVID MICHAEL · 2020 to 2024
$1.7M
Technology for evaluating drug-binding responses to small-molecule perturbationR35GM150919 · NIGMS · UNIVERSITY OF WASHINGTON · PI Devin Karl Schweppe · 2023 to 2026
$1.6M
A NanoLC-Orbitrap Tribrid Instrument for Comprehensive Proteomics AnalysesS10OD021502 · OD · UNIVERSITY OF WASHINGTON · PI ONG, SHAO-EN · 2017 to 2017
$906k
Leica SP8 WWL Confocal MicroscopeS10OD016240 · OD · UNIVERSITY OF WASHINGTON · PI CATTERALL, WILLIAM A · 2014 to 2014
$581k
NHLBI NIH HHS R01 HL160825NIGMS NIH HHS R01 GM138799NIGMS NIH HHS R35 GM137916NIGMS NIH HHS R35 GM150919NIGMS NIH HHS T32 GM007750NIH HHS S10 OD016240NIH HHS S10 OD021502
6 · The paper itself

Abstract

Eukaryotic nuclei adopt a highly compartmentalized architecture that influences nearly all genomic processes. Understanding how this architecture impacts gene expression has been hindered by a lack of tools for elucidating the molecular interactions at individual genomic loci. Here, we adapt oligonucleotide-mediated proximity-interactome mapping (O-MAP) to biochemically characterize discrete, micron-scale nuclear neighborhoods. By targeting O-MAP to introns within the

Identifiers

PMID39574693
PMCPMC11580901

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.