ArticleFungal biology and biotechnology2024
CRISPR-Cas9-mediated enhancement of Beauveria bassiana virulence with overproduction of oosporein.
Article in Fungal biology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Advances in Micro- and Macrobiological Strategies for Pest Control in Berry Production Systems: A Critical Review.Plants (Basel, Switzerland) · 2026Review
- Providing a toolbox for genomic engineering ofMicrobiology spectrum · 2025Article
- Molecular Strategies to Overcome Fungal Virulence in Crop Protection.Microbial biotechnology · 2025Review
- Transcription Factors in Biocontrol Fungi.Journal of fungi (Basel, Switzerland) · 2025Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Biocontrol agents play a pivotal role in managing pests and contribute to sustainable agriculture. Recent advancements in genetic engineering can facilitate the development of entomopathogenic fungi with desired traits to enhance biocontrol efficacy. In this study, a CRISPR-Cas9 ribonucleoprotein system was utilized to genetically improve the virulence of Beauveria bassiana, a broad-spectrum insect pathogen used in biocontrol of arthropod pests worldwide. CRISPR-Cas9-based disruption of the transcription factor-encoding gene Bbsmr1 led to derepression of the oosporein biosynthetic gene cluster resulting in overproduction of the red-pigmented dibenzoquinone oosporein involved in host immune evasion, thus increasing fungal virulence. Mutants defective for Bbsmr1 displayed a remarkable enhanced insecticidal activity by reducing lethal times and concentrations, while concomitantly presenting negligible or minor pleiotropic effects. In addition, these mutants displayed faster germination on the insect cuticle which correlated with higher density of free-floating blastospores in the hemolymph and accelerated mortality of the host. These findings emphasize the utility of genetic engineering in developing enhanced fungal biocontrol agents with customized phenotypic traits, and provide an efficient and versatile genetic transformation tool for application in other beneficial entomopathogenic fungi.
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Registered trials
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