Evidence map›Paper›PMID 39574085›Full record

ArticleBiomedical engineering online2024

Streamlined miRNA loading of surface protein-specific extracellular vesicle subpopulations through electroporation.

Corinna Torabi, Sung-Eun Choi, Thomas R Pisanic, Michael Paulaitis, Soojung Claire Hur

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical engineering online, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

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  14. Extracellular Vesicle-Based Drug Delivery Systems in Cancer Therapy.International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Corinna TorabiDepartment of Mechanical Engineering, Johns Hopkins University, 3400 N Charles Street, Baltimore, MD, 21218, USA.
Sung-Eun ChoiDepartment of Mechanical Engineering, Johns Hopkins University, 3400 N Charles Street, Baltimore, MD, 21218, USA.
Thomas R PisanicInstitute for NanoBioTechnology, Johns Hopkins University, 3400 N Charles Street, Baltimore, MD, 21218, USA.
Michael PaulaitisCenter for Nanomedicine at Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Soojung Claire HurDepartment of Mechanical Engineering, Johns Hopkins University, 3400 N Charles Street, Baltimore, MD, 21218, USA. schur@jhu.edu.

Funding

Development of a microfluidic primary cell editing platform (pCEP) for personal gene therapyR21CA229024 · NCI · JOHNS HOPKINS UNIVERSITY · PI HUR, SOOJUNG CLAIRE · 2020 to 2022
$551k
STEADI:System for Translational Exosome Analysis and Diagnostics through Integrating electroporation and single-molecule detectionR21TR005063 · NCATS · JOHNS HOPKINS UNIVERSITY · PI HUR, SOOJUNG CLAIRE · 2024 to 2025
$424k
National Science Foundation 1804004NCATS NIH HHS R21 TR005063NCI NIH HHS R21 CA229024NIH HHS R21CA229024NIH HHS R21TR005063
6 · The paper itself

Abstract

backgroundExtracellular vesicles (EVs) have emerged as an exciting tool for targeted delivery of therapeutics for a wide range of diseases. As nano-scale membrane-bound particles derived from living cells, EVs possess inherent capabilities as carriers of biomolecules. However, the translation of EVs into viable therapeutic delivery vehicles is challenged by lengthy and inefficient processes for cargo loading and pre- and post-loading purification of EVs, resulting in limited quantity and consistency of engineered EVs.

resultsIn this work, we develop a fast and streamlined method to load surface protein-specific subpopulations of EVs with miRNA by electroporating EVs, while they are bound to antibody-coated beads. We demonstrate the selection of CD81

conclusionsOur streamlined method integrates magnetic bead-based selection with electroporation, enabling rapid and efficient loading of miRNA into CD81

Indexed as

ElectroporationExtracellular VesiclesMicroRNAsHumansMembrane ProteinsTetraspanin 28Membrane ProteinsMicroRNAsTetraspanin 28ElectroporationEngineered extracellular vesiclesExtracellular vesiclesImmunopurificationmiRNA loadingmiRNA therapeuticsTargeted gene delivery

Identifiers

PMID39574085
PMCPMC11580418

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.