Evidence map›Paper›PMID 39574024›Full record

ArticleBMC microbiology2024

Chemical quantification of N-acyl alanine methyl ester (NAME) production and impact on temporal gene expression patterns in Roseovarius tolerans EL-164.

Janina Leinberger, Diana Koteska, Judith Boldt, Jörn Petersen, Sahana Shivaramu, Jürgen Tomasch, Stefan Schulz, Thorsten Brinkhoff

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In one paragraph

Article in BMC microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Janina LeinbergerInstitute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany. janina.leinberger@gmx.de.
Diana KoteskaInstitute of Organic Chemistry, Technische Universität Braunschweig, Braunschweig, Germany.
Judith BoldtLeibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Jörn PetersenLeibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Sahana ShivaramuLaboratory of Anoxygenic Phototrophs, Institute of Microbiology of the Czech Acad Sci, Třeboň, 37981, Czechia.
Jürgen TomaschLaboratory of Anoxygenic Phototrophs, Institute of Microbiology of the Czech Acad Sci, Třeboň, 37981, Czechia.
Stefan SchulzInstitute of Organic Chemistry, Technische Universität Braunschweig, Braunschweig, Germany.
Thorsten BrinkhoffInstitute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany. t.brinkhoff@icbm.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPrevious studies have identified structurally diverse N-acyl amino acid methyl esters (NAMEs) in culture extracts of Roseovarius tolerans EL-164 (Roseobacteraceae). NAMEs are structural analogues of the common signaling compounds N-acyl homoserine lactones (AHLs), but do not participate in AHL-mediated signaling. NAMEs show minor antialgal and antimicrobial activity, but whether this activity serves as the primary ecological role remains unclear.

resultsTo enable dose-dependent bioactivity-testing, we have established a chromatographic method for quantification of NAMEs in bacterial culture extracts. The concentrations determined for the two major NAMEs produced by EL-164, C16:1-NAME and C17:1-NAME, ranged between 0.685 and 5.731 mg L

conclusionsOur findings support previous studies suggesting an ecological role for C16:1-NAME as antibiotic, by proving that NAME concentrations in batch cultures were higher than the minimal inhibitory concentrations against Maribacter sp. 62 - 1 (Flavobacteriia) and Skeletonema costatum CCMP 1332 (Coscinodiscophyceae) reported in the literature. Our study further exemplified the broad application range of dose-dependent testing and highlighted the different biological activities of NAMEs and AHLs.

Indexed as

Gene Expression Regulation, BacterialAcyl-ButyrolactonesAlanineEstersRhodobacteraceaeAcyl-ButyrolactonesAlanineEstersAntibiosisDifferential gene expressionGrowth phasesQuantificationRoseobacterSecondary metabolitesSignalingTime courseTranscriptomics

Identifiers

PMID39574024
PMCPMC11580390

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.