Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha-4,11-Diene Production.

Carlos Castillo-Saldarriaga, Christine N S Santos, Stephen Sarria, Parayil K Ajikumar, Ralf Takors

Abstract read

In one paragraph

Article in Microbial biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Carlos Castillo-SaldarriagaInstitute of Biochemical Engineering, University of Stuttgart, Stuttgart, Germany.ORCID 0000-0003-0599-2996

Christine N S SantosManusBio, Cambridge, Massachusetts, USA.

Stephen SarriaManusBio, Cambridge, Massachusetts, USA.

Parayil K AjikumarManusBio, Cambridge, Massachusetts, USA.

Ralf TakorsInstitute of Biochemical Engineering, University of Stuttgart, Stuttgart, Germany.ORCID 0000-0001-5837-6906

Funding

ManusBio

6 · The paper itself

Abstract

Yeast extract (YE) is a complex nutritional source associated with high performance on microbial production processes. However, its inherent compositional variability challenges its scalability. While prior efforts have focused on growth-associated products, the dynamics of growth-uncoupled production, which leads to higher production rates and conversion yields, still need to be explored. This production scenario is common in large-scale applications. This study presents a systematic approach to replace YE for the production of the terpene amorpha-4,11-diene in Escherichia coli. Sequential processing was successfully applied to identify glutamic acid, alanine, leucine, valine, isoleucine and glycine as the key amino acids (AAs) under slow-growth conditions. Thoroughly applying biomass retention as part of sequential processing increased production capacity by 45% using these AAs instead of YE. Further studies, including flux balance analyses, targeted pyruvate as the common AA precursor. The optimized fed-batch process feeding pyruvate with 0.09 g

Indexed as

Culture MediaEscherichia coliPolycyclic SesquiterpenesAmino AcidsBiomassMetabolic Flux AnalysisPyruvic AcidSesquiterpenesAmino Acidsamorpha-4,11-dieneCulture MediaPolycyclic SesquiterpenesPyruvic AcidSesquiterpenesamino acidscomplex mediaEscherichia colifermentationpyruvateterpenoids

Identifiers

PMID39570580

DOI10.1111/1751-7915.70056

PMCPMC11580704

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.