Evidence map›Paper›PMID 39567611›Full record

ArticleScientific reports2024

Optimized simple culture protocol for inducing mature myotubes from MYOD1-overexpressed human iPS cells.

Eiji Wada, Nao Susumu, Yuya Okuzaki, Akitsu Hotta, Hidetoshi Sakurai, Yukiko K Hayashi

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In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Eiji WadaDepartment of Pathophysiology, Tokyo Medical University, Tokyo, Japan.
Nao SusumuDepartment of Pathophysiology, Tokyo Medical University, Tokyo, Japan.
Yuya OkuzakiCenter for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
Akitsu HottaCenter for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
Hidetoshi SakuraiCenter for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
Yukiko K HayashiDepartment of Pathophysiology, Tokyo Medical University, Tokyo, Japan. yhayashi@tokyo-med.ac.jp.

Funding

Acceleration Program for Intractable Disease Research Utilizing Disease-specific iPS cells JP17bm0804005Follow-up Grant from Tokyo Medical University 2022-2024Intramural Research Grant for Neurological and Psychiatric Disorders of NCNP 29-4KAKENHI 20H03594Practical Research Project for Rare/Intractable Diseases 23ek0109639h0001
6 · The paper itself

Abstract

The forced expression system of MYOD1, a master gene for myogenic differentiation, can efficiently and rapidly reproduce muscle differentiation of human induced pluripotent stem cells (hiPSCs). Despite these advantages of the MYOD1 overexpression system, developed myotubes are relatively immature and do not recapitulate several aspects of striated muscle fibers. Here, we developed a simple optimized protocol using an alternative culture medium for maximizing the advantages of the MYOD1 overexpression system, and successfully improved the formation of multinucleated mature myotubes within 10 days. In this study, we generated hiPSCs derived from healthy donors and an individual with congenial muscular dystrophy caused by LMNA mutation (laminopathy), and compared disease-associated phenotypes in differentiated myotubes generated by the conventional method and by our new optimized culture method. Using our optimized method, abnormal myonuclear shape was pronounced in the patient-derived iPSCs. In addition, abnormal accumulation of the nuclear membrane protein emerin was observed in LMNA-mutant hiPSCs. Our new culture method is expected to be widely applicable as a MYOD1 overexpression model of hiPSC-derived skeletal muscle cells for the analysis of a variety of muscle diseases.

Indexed as

Cell DifferentiationInduced Pluripotent Stem CellsMuscle Fibers, SkeletalMyoD ProteinCell Culture TechniquesCells, CulturedHumansLamin Type AMuscle DevelopmentMutationLamin Type AMyoD1 myogenic differentiation proteinMyoD ProteinDifferentiation of skeletal muscle cellsInduced pluripotent stem cellsLaminopathyMuscular dystrophyNewly developed culture method

Identifiers

PMID39567611
PMCPMC11579357

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.