Evidence map›Paper›PMID 39567421›Full record

ArticleGenes & genomics2025

Identification and expression analysis of the SPL gene family during flower bud differentiation in Rhododendron molle.

Dongmei Zhu, Xingmin Geng, Fanyu Zeng, Shida Xu, Jieyu Peng

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Article in Genes & genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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2 citing papers in PubMed.

  1. A review ofFrontiers in pharmacology · 2026
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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Dongmei ZhuCollege of Landscape Architecture, Nanjing Forestry University, Nanjing, 210037, China.
Xingmin GengCollege of Landscape Architecture, Nanjing Forestry University, Nanjing, 210037, China. xmgeng76@163.com.
Fanyu ZengCollege of Landscape Architecture, Nanjing Forestry University, Nanjing, 210037, China.
Shida XuCollege of Landscape Architecture, Nanjing Forestry University, Nanjing, 210037, China.
Jieyu PengCollege of Landscape Architecture, Nanjing Forestry University, Nanjing, 210037, China.

Funding

Jiangsu Province Innovation and Extension Project of Forestry Science and Technology (LYKJ [2024] 14)Priority Academic Program Development of Jiangsu Higher Education Institutions (Grant No.164120281)
6 · The paper itself

Abstract

backgroundThe family of SQUAMOSA promoter binding protein-like (SPL) transcription factors is essential for regulating plant growth and development. While this SPL gene functional research has been limited in Rhododendron molle (R. molle).

objectiveTo preliminarily explore the regulatory mechanism of the SPL gene in flower bud development of R. molle.

methodsIn this study, for R. molle, the flower bud differentiation period was determined by observing the morphological anatomy of the flower bud. The SPL gene family members were identified based on the R. molle genome, Additionally, the expressions of RmSPL genes at five flower bud differentiation stages were analyzed via Quantitative reverse transcription PCR (RT-qPCR).

resultsWe first characterized 20 SPL family members in the reference genome of R. molle. The phylogenetic analysis of plant SPL proteins separated them into eight subfamilies (G1-G8) according to conserved gene structures and protein motifs. Cis-elements of promoter region analysis showed that RmSPL genes were regulated by light, phytohormones, stress response, and plant growth and development and may play a critical role in the photoresponse, abasic acid, anaerobic induction, and meristematic expression. Gene expression analysis showed that 18 RmSPL genes were differentially expressed in different developing flower buds. In particular, RmSPL1/7/8/12/13 exhibited significantly different expressions, suggesting that they were likely essential genes for regulating the differentiation of flower buds.

conclusionIn conclusion, our analysis of RmSPL genes provides a theoretical basis and reference for future functional analysis of RmSPL genes in the flower bud differentiation of R. molle.

Indexed as

FlowersPlant ProteinsRhododendronTranscription FactorsGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantMultigene FamilyPhylogenyPromoter Regions, GeneticPlant ProteinsTranscription FactorsBioinformatics analysisExpression analysisFlower bud differentiationRhododendron molleSPL gene family

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.