Evidence map›Paper›PMID 39566904›Full record

ArticleACS infectious diseases2024

Studying Target-Engagement of Anti-Infectives by Solvent-Induced Protein Precipitation and Quantitative Mass Spectrometry.

Lorenzo Bizzarri, Dominik Steinbrunn, Thibaut Quennesson, Antoine Lacour, Gabriella Ines Bianchino, Patricia Bravo, Philippe Chaignon, Jonas Lohse, Pascal Mäser, Myriam Seemann and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS infectious diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Lorenzo BizzarriOmicScouts GmbH, Lise-Meitner-Straße 30, Freising D-85354, Germany.ORCID 0009-0003-7017-5770
Dominik SteinbrunnOmicScouts GmbH, Lise-Meitner-Straße 30, Freising D-85354, Germany.
Thibaut QuennessonLaboratory for Medicinal Chemistry (Campus Heymans), Ghent University, Ottergemsesteenweg 460, Gent B-9000, Belgium.
Antoine LacourDepartment of Pharmacy, Saarland University, Campus E8.1, Saarbrücken D-66123, Germany.
Gabriella Ines BianchinoEquipe Chimie Biologique et Applications Thérapeutiques, Institut de Chimie de Strasbourg, UMR 7177, Université de Strasbourg/CNRS, 4, Rue Blaise Pascal, Strasbourg F-67070, France.
Patricia BravoSwiss Tropical and Public Health Institute (Swiss TPH), Kreuzstrasse 2, Allschwil CH-4123, Switzerland.ORCID 0000-0001-5454-3826
Philippe ChaignonEquipe Chimie Biologique et Applications Thérapeutiques, Institut de Chimie de Strasbourg, UMR 7177, Université de Strasbourg/CNRS, 4, Rue Blaise Pascal, Strasbourg F-67070, France.
Jonas LohseOmicScouts GmbH, Lise-Meitner-Straße 30, Freising D-85354, Germany.
Pascal MäserSwiss Tropical and Public Health Institute (Swiss TPH), Kreuzstrasse 2, Allschwil CH-4123, Switzerland.ORCID 0000-0003-3122-1941
Myriam SeemannEquipe Chimie Biologique et Applications Thérapeutiques, Institut de Chimie de Strasbourg, UMR 7177, Université de Strasbourg/CNRS, 4, Rue Blaise Pascal, Strasbourg F-67070, France.ORCID 0000-0002-2615-1574
Serge Van CalenberghLaboratory for Medicinal Chemistry (Campus Heymans), Ghent University, Ottergemsesteenweg 460, Gent B-9000, Belgium.ORCID 0000-0002-4201-1264
Anna K H HirschDepartment of Pharmacy, Saarland University, Campus E8.1, Saarbrücken D-66123, Germany.ORCID 0000-0001-8734-4663
Hannes HahneOmicScouts GmbH, Lise-Meitner-Straße 30, Freising D-85354, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antimicrobial resistance (AMR) poses a serious threat to global health. The rapid emergence of resistance contrasts with the slow pace of antimicrobial development, emphasizing the urgent need for innovative drug discovery approaches. This study addresses a critical bottleneck in early drug development by introducing integral solvent-induced protein precipitation (iSPP) to rapidly assess the target-engagement of lead compounds in extracts of pathogenic microorganisms under close-to-physiological conditions. iSPP measures the change in protein stability against solvent-induced precipitation in the presence of ligands. The iSPP method for bacteria builds upon established SPP procedures and features optimized denaturation gradients and minimized sample input amounts. The effectiveness of the iSPP workflow was initially demonstrated through a multidrug target-engagement study. Using quantitative mass spectrometry (LC-MS/MS), we successfully identified known drug targets of seven different antibiotics in cell extracts of four AMR-related pathogens: the three Gram-negative bacteria

Indexed as

SolventsTandem Mass SpectrometryAnti-Bacterial AgentsAnti-Infective AgentsBacterial ProteinsChromatography, LiquidDrug DiscoveryEscherichia coliKlebsiella pneumoniaeMicrobial Sensitivity TestsPseudomonas aeruginosaStaphylococcus aureusAnti-Bacterial AgentsAnti-Infective AgentsBacterial ProteinsSolventsantibioticsmass spectrometryMEP pathwayproteomicssolvent-induced precipitationtarget identification

Identifiers

PMID39566904
PMCPMC11650766

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.