Evidence map›Paper›PMID 39565583›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Identification of Small-Molecule Modulators of FOXO3 Through Virtual Screening.

Filoemena Nascimento, Gonçalo Braga, Paulo J Martel

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Filoemena NascimentoCINTESIS@RISE/Faculty of Sciences and Technology, University of the Algarve, Faro, Portugal.
Gonçalo BragaCINTESIS@RISE/Faculty of Sciences and Technology, University of the Algarve, Faro, Portugal.
Paulo J MartelCINTESIS@RISE/Faculty of Sciences and Technology, University of the Algarve, Faro, Portugal. pmartel@ualg.pt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

FOXO3 is integral in regulating numerous genes involved in critical cellular processes such as apoptosis, oxidative damage protection, cell growth, and cancer. Consequently, modulating FOXO3 activity holds significant potential for applications in cancer treatment and cellular aging. A promising approach involves identifying small-molecule modulators that can either enhance or inhibit FOXO3's DNA-binding capability. This paper details a virtual screening protocol aimed at discovering such modulators. Utilizing the crystal structures of FOXO3 in both its free and DNA-bound forms, we pinpoint potential binding sites that may disrupt or facilitate the DNA-FOXO3 interaction. A comprehensive virtual screening of a small-molecule compound library is conducted using AutoDock Vina software. The highest-ranking hits for each site are carefully selected and analyzed to determine their binding modes. This protocol paves the way for identifying novel modulators of FOXO3, offering therapeutic avenues in cancer and aging-related research.

Indexed as

Forkhead Box Protein O3Small Molecule LibrariesBinding SitesDNADrug Evaluation, PreclinicalHumansMolecular Docking SimulationProtein BindingSoftwareDNAForkhead Box Protein O3FOXO3 protein, humanSmall Molecule LibrariesDockingDrug designFOXOSmall moleculeTranscription factorVirtual screening

Identifiers

PMID39565583

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.