ArticleThe EMBO journal1986
Selection of Dictyostelium mutants defective in cytoskeletal proteins: use of an antibody that binds to the ends of alpha-actinin rods.
Article in The EMBO journal, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 127 citations in OpenAlex.
- Actin-Interacting Protein 1 Contributes to Intranuclear Rod Assembly in Dictyostelium discoideum.Scientific reports · 2017Article
- A Simple Retroelement Based Knock-Down System in Dictyostelium: Further Insights into RNA Interference Mechanisms.PloS one · 2015Article
- Argonaute proteins affect siRNA levels and accumulation of a novel extrachromosomal DNA from the Dictyostelium retrotransposon DIRS-1.The Journal of biological chemistry · 2014Article
- Actin binding domains direct actin-binding proteins to different cytoskeletal locations.BMC cell biology · 2008Article
- CRP1, a LIM domain protein implicated in muscle differentiation, interacts with alpha-actinin.The Journal of cell biology · 1997Article
- Dictyostelium discoideum cells lacking the 34,000-dalton actin-binding protein can grow, locomote, and develop, but exhibit defects in regulation of cell structure and movement: a case of partial redundancy.The Journal of cell biology · 1996Article
- Mechanical perturbation elicits a phenotypic difference between Dictyostelium wild-type cells and cytoskeletal mutants.Biophysical journal · 1996Article
- Article
- Cell-substrate interactions and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins: a study using quantitative reflection interference contrast microscopy.Biophysical journal · 1995Article
- Dictyostelium discoideum mutants with conditional defects in phagocytosis.The Journal of cell biology · 1994Article
- Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin.The Biochemical journal · 1994Article
- Determination of the alpha-actinin-binding site on actin filaments by cryoelectron microscopy and image analysis.The Journal of cell biology · 1994Article
- Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motility.The Journal of cell biology · 1993Article
- An interaction between zyxin and alpha-actinin.The Journal of cell biology · 1992Article
- Perturbations of Drosophila alpha-actinin cause muscle paralysis, weakness, and atrophy but do not confer obvious nonmuscle phenotypes.The Journal of cell biology · 1992Article
- Targeted disruption of the ABP-120 gene leads to cells with altered motility.The Journal of cell biology · 1992Article
- The actin filament severing protein actophorin promotes the formation of rigid bundles of actin filaments crosslinked with alpha-actinin.The Journal of cell biology · 1991Article
- Coronin, an actin binding protein of Dictyostelium discoideum localized to cell surface projections, has sequence similarities to G protein beta subunits.The EMBO journal · 1991Article
- Vertebrate muscle Z-line structure: an electron microscopic study of negatively-stained myofibrils.Journal of muscle research and cell motility · 1991Article
- The three-dimensional structure of the nemaline rod Z-band.The Journal of cell biology · 1990Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A monoclonal antibody, mAb 47-19-2, was used to study the subunit topology of the rod-shaped alpha-actinin molecules of Dictyostelium discoideum and to screen for mutants defective in the production of alpha-actinin. Electron microscopy of rotary-shadowed alpha-actinin-antibody complexes showed binding of mAb 47-19-2 to both ends of the alpha-actinin rods and cleavage of the rods into its subunits, indicating that the two subunits of alpha-actinin extend in an anti-parallel mode through the whole length of the rod. The antibody binding sites were located in close proximity to the sites responsible for actin cross-linking, which is consistent with the blocking activity of the antibody. In a mutant, HG1130, no antibody label was detected in colony blots, and by immunoblotting of mutant proteins separated by SDS-PAGE, only trace amounts of alpha-actinin were found. The mutant showed normal binding of antibodies directed against the actin-binding proteins severin and capping protein. The mutation responsible for the alpha-actinin defect was recessive and located on linkage group I of the genetic map of D. discoideum. HG1130 cells grew on bacteria at a normal rate and also axenically like cells of the parent strain AX2. After starvation the mutant cells expressed the contact site A glycoprotein, a marker of the aggregation-competent stage, and reacted chemotactically to cyclic AMP. The aggregation patterns and fruiting bodies of the mutant appeared to be normal. Patching and capping on the surface of HG1130 cells was induced by antibodies against the contact site A glycoprotein.(ABSTRACT TRUNCATED AT 250 WORDS)
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