Evidence map›Paper›PMID 39564775›Full record

ArticleJournal of cell science2024

ImmunoCellCycle-ID - a high-precision immunofluorescence-based method for cell cycle identification.

Yu-Lin Chen, Yu-Chia Chen, Aussie Suzuki

Abstract read
In one paragraph

Article in Journal of cell science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. HIV-1 Vif and Vpr cooperatively modulate the cell cycle to maximize per-cell virion production.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Yu-Lin ChenMcArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Yu-Chia ChenMcArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Aussie SuzukiMcArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.ORCID 0000-0001-7390-5116

Funding

Center for Structural Biology of HIV RNAU54AI170660 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ALICE TELESNITSKY · 2022 to 2026
$32.1M
Chromosome dynamics and organizations necessary for faithful chromosome segregationR35GM147525 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Aussie Suzuki · 2022 to 2026
$2.5M
McArdle Laboratory for Cancer ResearchNIAID NIH HHS U54 AI170660NIGMS NIH HHS R35 GM147525NIH HHS R35GM147525University of Wisconsin-MadisonUniversity of Wisconsin-Madison Office of the Vice Chancellor for ResearchUW Carbone Cancer CenterWisconsin Alumni Research FoundationWisconsin Partnership Program
6 · The paper itself

Abstract

The cell cycle is a fundamental process essential for cell proliferation, differentiation and development. It consists of four major phases: G1, S, G2 and M. These phases collectively drive the reproductive cycle and are meticulously regulated by various proteins that play crucial roles in both the prevention and progression of cancer. Traditional methods for studying these functions, such as flow cytometry, require a substantial number of cells to ensure accuracy. In this study, we have developed a user-friendly immunofluorescence-based method for identifying cell cycle stages, providing single-cell resolution and precise identification of G1, early/mid S, late S, early/mid G2, late G2, and each sub-stage of the M phase using fluorescence microscopy called ImmunoCellCycle-ID. This method provides high-precision cell cycle identification and can serve as an alternative to, or in combination with, traditional flow cytometry to dissect detailed sub-stages of the cell cycle in a variety of cell lines.

Indexed as

Cell CycleFlow CytometryFluorescent Antibody TechniqueHumansMicroscopy, FluorescenceCell cycleImagingImmunofluorescence-based cell cycle identificationMicroscopy

Identifiers

PMID39564775
PMCPMC11607684

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.