Evidence map›Paper›PMID 39561265›Full record

ArticleFEBS open bio2025

Structural analysis of an Asterias rubens peptide indicates the presence of a disulfide-directed β-hairpin fold.

Rozita Takjoo, David T Wilson, Justine Le Quilliec, Casey A Schmidt, Guangzu Zhao, Michael J Liddell, Naeem Y Shaikh, Kartik Sunagar, Alex Loukas, Michael J Smout and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rozita TakjooAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.
David T WilsonAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.ORCID https://orcid.org/0000-0001-5047-0711
Justine Le QuilliecAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.ORCID https://orcid.org/0009-0000-7945-9575
Casey A SchmidtAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.
Guangzu ZhaoAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.
Michael J LiddellCollege of Science and Engineering, James Cook University, Cairns, Australia.ORCID https://orcid.org/0000-0001-9754-8184
Naeem Y ShaikhEvolutionary Venomics Lab, Centre for Ecological Sciences, Indian Institute of Science, Bangalore, India.
Kartik SunagarEvolutionary Venomics Lab, Centre for Ecological Sciences, Indian Institute of Science, Bangalore, India.
Alex LoukasAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.
Michael J SmoutAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.
Norelle L DalyAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.

Funding

Targeting parasite-host communication to combat liver fluke-induced bile duct cancerR01CA164719 · NCI · GEORGE WASHINGTON UNIVERSITY · PI Paul J Brindley, THEWARACH LAHA · 2012 to 2026
$5.0M
Australian Research Council FF110100226Australian Research Council LE160100218James Cook UniversityMerchant FoundationNCI NIH HHS R01 CA164719NIH HHS R01CA164719Wellcome TrustWellcome Trust DBT India Alliance IA/I/19/2/504647
6 · The paper itself

Abstract

Sea stars are an abundant group of marine invertebrates that display remarkably robust regenerative capabilities throughout all life stages. Numerous proteins and peptides have been identified in a proteome study on the coelomic fluid (biofluid) of the common sea star Asterias rubens, which appear to be involved with the wound-healing response in the organism. However, the three-dimensional structure and function of several of these injury-responsive peptides, including the peptide KASH2, are yet to be investigated. Here, we show that the KASH2 peptide adopts a disulfide-directed β-hairpin fold (DDH). The DDH motif appears to be evolutionarily related to the inhibitor cystine knot motif, which is one of the most widespread disulfide-rich peptide folds. The DDH motif was originally thought to be restricted to arachnids, but our study suggests that as a result of convergent evolution it could also have originated in sea stars. Although the widely conserved DDH fold has potential cross-phyla wound-healing capacity, we have shown that KASH2 does not enhance the proliferation of human fibroblasts, a simple method for wound-healing re-epithelialisation screening. Therefore, additional research is necessary to determine the role of KASH2 in the sea stars.

Indexed as

AsteriasDisulfidesPeptidesAmino Acid SequenceAnimalsCell ProliferationFibroblastsHumansProtein FoldingWound HealingDisulfidesPeptidescell proliferation assaydisulfide‐directed β‐hairpinmolecular evolutionNMR spectroscopypeptide synthesis

Identifiers

PMID39561265
PMCPMC11891777

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.