Alterations in Circular RNAs circOprm1 and circSerpini in the Striatum are Associated with Changes in Spatial Working Memory Performance after Morphine Dependence and Withdrawal in Rats.

Shamseddin Ahmadi, Abdulbaset Vali, Samira Amiri, Danesh Rostami, Mohammad Majidi, Karim Rahimi

Abstract read

In one paragraph

Article in Neurochemical research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 · What the graph read from it

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1 citing paper in PubMed.

The Effect of Vitamin D3 Injection Combined With High-Intensity Interval Training on Excessive Autophagy in the Heart Tissue of Type 2 Diabetes-Induced Rats: An Analysis of the mTOR-Beclin-1-Fyco-1-Cathepsin D Pathway.Cardiovascular therapeutics · 2025
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5 · Who and what money

Authors and funding

6 authors.

Shamseddin AhmadiDepartment of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Iran. sh.ahmadi@uok.ac.ir.ORCID http://orcid.org/0000-0003-0300-3226

Abdulbaset ValiDepartment of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Iran.

Samira AmiriDepartment of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Iran.

Danesh RostamiDepartment of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Iran.

Mohammad MajidiDepartment of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Iran.

Karim RahimiDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.

Funding

the Vice Chancellorship of Research and Innovation at the University of Kurdistan 1399

6 · The paper itself

Abstract

Modulating role of circRNAs and microRNAs in neurobiological changes induced by drug exposure remains unclear. We examined alterations in some circRNAs and microRNAs in the striatum after morphine dependence and withdrawal and their associations with the changes in spatial working memory performance. Male Wistar rats were used in which 10 days morphine exposure induced dependence. Withdrawal effects were assessed 30 days after stopping morphine exposure. Spatial working memory was assessed using a Y maze test on days 1 and 10 of the drug exposure and 30 days after withdrawal. The gene and protein expression were assessed after dependence and withdrawal. The results revealed that 10 days morphine exposure impaired working memory, which partially reinstated after withdrawal. After 10 days morphine exposure, significant increases in Oprm1 gene and OPRM1 protein levels were detected, which persisted even after withdrawal. The expression of circOprm1 and miR-339-3p decreased in the morphine-dependent group, but they returned to normal levels after withdrawal. The expression of Tlr4 gene and TLR4 protein levels decreased after dependence. While Tlr4 mRNA levels returned to normal after withdrawal, TLR4 protein levels remained lower than the control group. In the morphine-dependent group, both Serpini1 and circSerpini expression significantly increased, but they restored after withdrawal. Expression of miR-181b-3p, miR-181b-5p, miR-181c-3p, and miR-181c-5p decreased after dependence, but they reinstated after withdrawal. It can be concluded that circOprm1 and circSerpini via regulating the OPRM1 and TLR4 expression in the striatum are associated with the neuroadaptation underlying spatial working memory after both morphine dependence and withdrawal.

Indexed as

Corpus StriatumMicroRNAsMorphine DependenceRats, WistarReceptors, Opioid, muRNA, CircularSpatial MemorySubstance Withdrawal SyndromeAnimalsMaleMaze LearningMemory, Short-TermMorphineRatsToll-Like Receptor 4MicroRNAsMorphineOprm1 protein, ratReceptors, Opioid, muRNA, CircularTlr4 protein, ratToll-Like Receptor 4Circular RNAsmicroRNAsMorphine dependenceStriatumWithdrawalWorking memory

Identifiers

PMID39560876

DOI10.1007/s10753-019-01049-3

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.