ArticlePlanta2024
The origin and metabolic fate of 4-hydroxybenzoate in Arabidopsis.
Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Multi-omics profiling of microbial ecology and non-volatile compounds across fermentation stages of spontaneous litchi (Frontiers in nutrition · 2026Article
- Integrated metabolomic and transcriptomic analysis reveals variation in the metabolites and genes of Sanghuangporus sanghuang from Sanghuang-related fungi.Archives of microbiology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
MAIN
conclusionThe contribution of p-coumarate β-oxidation and kaempferol cleavage to the pools of glycosylated, free and cell wall-bound 4-hydroxybenzoate is organ-dependent in Arabidopsis. 4-Hydroxybenzoate (4-HB) is a vital precursor for a number of plant primary and specialized metabolites, as well as for the assembly of the plant cell wall. In Arabidopsis, it is known that 4-HB is derived independently from phenylalanine and tyrosine, and that the metabolism of phenylalanine into 4-HB proceeds via at least two biosynthetic routes: the β-oxidation of p-coumarate and the peroxidative cleavage of kaempferol. The precise contribution of these precursors and branches to 4-HB production, however, is not known. Here, we combined isotopic feeding assays, reverse genetics, and quantification of soluble (i.e., free and glycosylated) and cell wall-bound 4-HB to determine the respective contributions of phenylalanine, tyrosine, β-oxidation of p-coumarate, and peroxidative cleavage of kaempferol to 4-HB biosynthesis in Arabidopsis tissues. Over 90% of 4-HB was found to originate from phenylalanine in both leaves and roots. Soluble 4-HB level varied significantly between organs, while the proportion of cell wall-bound 4-HB was relatively constant. In leaves and flowers, glycosylated and cell wall-bound 4-HB were the most and least abundant forms, respectively. Flowers displayed the highest specific content of 4-HB, while free 4-HB was not detected in roots. Although p-coumarate β-oxidation and kaempferol catabolism were found to both contribute to the supply of 4-HB in all tissues, the proportion of kaempferol-derived 4-HB was higher in roots than in leaves and flowers. Within the β-oxidative branch, p-coumaroyl-CoA ligase 4-CL8 (At5g38120) bore a preponderant role in the production of soluble and cell wall-bound 4-HB in leaves, while p-coumaroyl-CoA ligase At4g19010 appeared to control the biosynthesis of soluble 4-HB in flowers. Furthermore, analysis of a series of Arabidopsis T-DNA mutants corresponding to the three major UDP-glucosyltransferases known to act on 4-HB in vitro (UGT75B1, UGT89B1, and UGT71B1) showed that none of these enzymes appeared in fact to have a significant role in the glycosylation of 4-HB in vivo.
Indexed as
Identifiers
39560789What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.