Evidence map›Paper›PMID 39560778›Full record

ArticleApplied microbiology and biotechnology2024

Biocatalytic sulfation of aromatic and aliphatic alcohols catalyzed by arylsulfate sulfotransferases.

Isabel Oroz-Guinea, Marko Rath, Isabelle Tischler, Klaus Ditrich, Doreen Schachtschabel, Michael Breuer, Wolfgang Kroutil

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Isabel Oroz-GuineaAustrian Centre of Industrial Biotechnology C/o University of Graz, Heinrichstrasse 28, 8010, Graz, Austria.
Marko RathAustrian Centre of Industrial Biotechnology C/o University of Graz, Heinrichstrasse 28, 8010, Graz, Austria.
Isabelle TischlerInstitute of Chemistry, University of Graz, NAWI Graz, Heinrichstrasse 28, 8010, Graz, Austria.
Klaus DitrichWhite Biotechnology Research, BASF SE, Carl-Bosch-Strasse 38, 67056, Ludwigshafen, Germany.
Doreen SchachtschabelWhite Biotechnology Research, BASF SE, Carl-Bosch-Strasse 38, 67056, Ludwigshafen, Germany.
Michael BreuerWhite Biotechnology Research, BASF SE, Carl-Bosch-Strasse 38, 67056, Ludwigshafen, Germany.
Wolfgang KroutilAustrian Centre of Industrial Biotechnology C/o University of Graz, Heinrichstrasse 28, 8010, Graz, Austria. wolfgang.kroutil@uni-graz.at.ORCID http://orcid.org/0000-0002-2151-6394

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many relevant metabolites, as well as chemical commodities, contain at least one sulfate ester group. Consequently, biocatalytic strategies to attach sulfate to a molecule under mild conditions are of high interest. In order to expand the enzymatic toolbox available, five new arylsulfate sulfotransferases (ASSTs) were identified in this study. Overexpression in Escherichia coli and enzyme purification resulted in soluble proteins which catalyzed the sulfate transfer to an acceptor substrate using p-nitrophenyl sulfate (pNPS) as sulfate donor. Optimal reaction conditions were established with respect to temperature and pH, as well as their tolerance to organic co-solvents and melting temperature. Additionally, the kinetic parameters (V

Indexed as

AlcoholsArylsulfotransferaseBiocatalysisEscherichia coliAniline CompoundsHydrogen-Ion ConcentrationKineticsNitrobenzenesNitrophenolsSubstrate SpecificitySulfatesTemperature4-nitrophenyl sulfateAlcoholsanilineAniline CompoundsArylsulfotransferaseNitrobenzenesNitrophenolsSulfatesAliphatic alcohol sulfationAmine sulfationArylsulfate sulfotransferaseDisulfated compoundsEnzymatic sulfation

Identifiers

PMID39560778
PMCPMC11576661

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.