Evidence map›Paper›PMID 39559312›Full record

ArticleInternational journal of ophthalmology2024

Cone-rod homeobox transcriptionally activates TCF7 to promote the proliferation of retinal pigment epithelial and retinoblastoma cells

Na Zhao, Ying-Ying Li, Jia-Man Xu, Mu-Yao Yang, Yun-Zhe Li, Thomas Chuen Lam, Lei Zhou, Qi-Hu Tong, Jun-Tao Zhang, Sheng-Zhan Wang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of ophthalmology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Na ZhaoOphthalmology Center, the Affiliated People's Hospital of Ningbo University, Ningbo 315040, Zhejiang Province, China.
Ying-Ying LiOphthalmology Center, the Affiliated People's Hospital of Ningbo University, Ningbo 315040, Zhejiang Province, China.
Jia-Man XuReproductive Medicine Center, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Mu-Yao YangCollege of Bioengineering, Chongqing University, Chongqing 400044, China.
Yun-Zhe LiDepartment of Gynaecology and Obstetrics, the First Affiliated Hospital of Chongqing Medical University; Department of Gynecology, The First Branch of The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042; State Key Laboratory of Maternal and Fetal Medicine of Chongqing, Chongqing Medical University, Chongqing 400016, China.
Thomas Chuen LamCentre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong 999077, China.
Lei ZhouCentre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong 999077, China.
Qi-Hu TongOphthalmology Center, the Affiliated People's Hospital of Ningbo University, Ningbo 315040, Zhejiang Province, China.
Jun-Tao ZhangOphthalmology Center, the Affiliated People's Hospital of Ningbo University, Ningbo 315040, Zhejiang Province, China.
Sheng-Zhan WangOphthalmology Center, the Affiliated People's Hospital of Ningbo University, Ningbo 315040, Zhejiang Province, China.
Xin-Xin HuOphthalmology Center, the Affiliated People's Hospital of Ningbo University, Ningbo 315040, Zhejiang Province, China.
Yu-Fei WuOphthalmology Center, the Affiliated People's Hospital of Ningbo University, Ningbo 315040, Zhejiang Province, China.
Qin-Kang LuOphthalmology Center, the Affiliated People's Hospital of Ningbo University, Ningbo 315040, Zhejiang Province, China.
Ting-Yuan LangReproductive Medicine Center, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo investigate the proliferation regulatory effect of cone-rod homeobox (CRX) in retinal pigment epithelium (RPE) and retinoblastoma (RB) cells to explore the potential application and side effect (oncogenic potential) of CRX-based gene therapy in RPE-based retinopathies.

methodsAdult human retinal pigment epithelial (ARPE)-19 and human retinal pigment epithelial (RPE)-1 cells and Y79 RB cell were used in the study. Genetic manipulation was performed by lentivirus-based technology. The cell proliferation was determined by a CellTiter-Glo Reagent. The mRNA and protein levels were determined by quantitative real-time polymerase chain reaction (qPCR) and Western blot assay. The transcriptional activity of the promoter was determined by luciferase reporter gene assay. The bindings between CRX and transcription factor 7 (TCF7) promoter as well as TCF7 and the promoters of TCF7 target genes were examined by chromatin immunoprecipitation (ChIP) assay. The transcription of the TCF7 was determined by a modified nuclear run-on assay.

resultsCRX overexpression and knockdown significantly increased (

conclusionCRX transcriptionally activates TCF7 to promote the proliferation of RPE and RB cells

Indexed as

cone-rod homeoboxregenerative medicineretinal pigment epithelial cellretinoblastomatranscription factor 7tumorigenic potential

Identifiers

PMID39559312
PMCPMC11528266

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