Evidence map›Paper›PMID 39558354›Full record

ArticleGenome biology2024

Transcription of a centromere-enriched retroelement and local retention of its RNA are significant features of the CENP-A chromatin landscape.

B J Chabot, R Sun, A Amjad, S J Hoyt, L Ouyang, C Courret, R Drennan, L Leo, A M Larracuente, L J Core and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. The dynamic centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025
    Review
  5. Fungi as models of centromere innovation: from DNA sequence to 3-dimensional arrangement.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025
    Review
  6. CENP-A and centromere evolution in equids.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025
    Review
  7. Article
  8. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

B J ChabotDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.
R Sun *Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.
A Amjad *Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.
S J Hoyt *Institute for Systems Genomics, University of Connecticut, Storrs, CT, USA.
L OuyangDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.
C CourretDepartment of Biology, University of Rochester, Rochester, NY, USA.
R DrennanDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.
L LeoDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.
A M LarracuenteDepartment of Biology, University of Rochester, Rochester, NY, USA.
L J CoreDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.
R J O'NeillDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.
B G MelloneDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA. Barbara.Mellone@uconn.edu.

Funding

Genetic and Epigenetic Determinants of Centromere IdentityR35GM131868 · NIGMS · UNIVERSITY OF CONNECTICUT STORRS · PI BARBARA MELLONE · 2019 to 2026
$3.1M
Regulation, evolution, and function of promoter-associated non-coding RNAsR35GM128857 · NIGMS · UNIVERSITY OF CONNECTICUT STORRS · PI CORE, LEIGHTON JAMES · 2018 to 2022
$2.0M
The Role of Retroelements in Centromere FunctionR01GM123312 · NIGMS · UNIVERSITY OF CONNECTICUT STORRS · PI O'NEILL, RACHEL · 2019 to 2022
$1.9M
NIGMS NIH HHS R01 GM123312NIGMS NIH HHS R35 GM128857NIGMS NIH HHS R35 GM131868
6 · The paper itself

Abstract

backgroundCentromeres depend on chromatin containing the conserved histone H3 variant CENP-A for function and inheritance, while the role of centromeric DNA repeats remains unclear. Retroelements are prevalent at centromeres across taxa and represent a potential mechanism for promoting transcription to aid in CENP-A incorporation or for generating RNA transcripts to maintain centromere integrity.

resultsIn this study, we probe into the transcription and RNA localization of the centromere-enriched retroelement G2/Jockey-3 (hereafter referred to as Jockey-3) in Drosophila melanogaster, currently the only in vivo model with assembled centromeres. We find that Jockey-3 is a major component of the centromeric transcriptome and produces RNAs that localize to centromeres in metaphase. Leveraging the polymorphism of Jockey-3 and a de novo centromere system, we show that these RNAs remain associated with their cognate DNA sequences in cis, suggesting they are unlikely to perform a sequence-specific function at all centromeres. We show that Jockey-3 transcription is positively correlated with the presence of CENP-A and that recent Jockey-3 transposition events have occurred preferentially at CENP-A-containing chromatin.

conclusionsWe propose that Jockey-3 preferentially inserts at the centromere to ensure its own selfish propagation, while contributing to transcription across these regions. Given the conservation of retroelements as centromere components through evolution, our findings may offer a basis for understanding similar associations in other species.

Indexed as

CentromereCentromere Protein AChromatinDrosophila melanogasterDrosophila ProteinsRetroelementsTranscription, GeneticAnimalsRNACentromere Protein AChromatinCid protein, DrosophilaDrosophila ProteinsRetroelementsRNA

Identifiers

PMID39558354
PMCPMC11575011

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.