Evidence map›Paper›PMID 39556480›Full record

ArticleG3 (Bethesda, Md.)2025

Bellymount-pulsed tracking: a novel approach for real-time in vivo imaging of Drosophila abdominal tissues.

Shruthi Balachandra, Amanda A Amodeo

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Shruthi BalachandraDepartment of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.ORCID 0000-0002-3286-1222
Amanda A AmodeoDepartment of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.ORCID 0000-0003-3701-6375

Funding

Understanding the role of RNA-binding protein mutations in cancerP20GM113132 · NIGMS · DARTMOUTH COLLEGE · PI MIERKE, DALE F · 2016 to 2025
$25.9M
The coordination of cell size control and cell cycle regulation at developmental extremesR35GM150853 · NIGMS · DARTMOUTH COLLEGE · PI Amanda A Amodeo · 2023 to 2026
$1.6M
NIGMS NIH HHS P20 GM113132NIGMS NIH HHS R35 GM150853NIH NIGMS COBRE P20-GM113132NIH NIGMS MIRA 1R35GM150853-01
6 · The paper itself

Abstract

Quantitative live imaging is a valuable tool that offers insights into cellular dynamics. However, many fundamental biological processes are incompatible with current live-imaging modalities. Drosophila oogenesis is a well-studied system that has provided molecular insights into a range of cellular and developmental processes. The length of the oogenesis, coupled with the requirement for inputs from multiple tissues, has made long-term culture challenging. Here, we have developed Bellymount-pulsed tracking (Bellymount-PT), which allows continuous, noninvasive live imaging of Drosophila oogenesis inside the female abdomen for up to 16 h. Bellymount-PT improves upon the existing Bellymount technique by adding pulsed anesthesia with periods of feeding that support the long-term survival of flies during imaging. Using Bellymount-PT, we measure key events of oogenesis, including egg chamber growth, yolk uptake, and transfer of specific proteins to the oocyte during nurse cell dumping with high spatiotemporal precision within the abdomen of a live female.

Indexed as

AbdomenOogenesisAnimalsDrosophilaDrosophila melanogasterFemaleOocytesdevelopmental dynamicsin vivo live imagingoogenesisprotein accumulation

Identifiers

PMID39556480
PMCPMC11708215

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.