ArticlePractical laboratory medicine2024
Development of a rapid LFA test based on direct RT-LAMP for diagnosis of SARS-CoV-2.
Article in Practical laboratory medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Trends of nucleic acid - based point-of-care diagnostics for infectious diseases.Journal of biological engineering · 2026Review
- Recent advances in lateral flow devices and point-of-care diagnostics for highly pathogenic avian influenza A viruses.Journal of virology · 2025Review
- Development of Visual Loop-Mediated Isothermal Amplification Assays for Foodborne Hepatitis A Virus.Foods (Basel, Switzerland) · 2025Article
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Authors and funding
6 authors.
Funding
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Abstract
Introduction: In response to the rapid spread of the SARS-CoV-2 virus, we developed a rapid molecular approach to diagnose COVID-19 without the need for RNA extraction. Methods: The study utilized two molecular methods, RT-qPCR and colorimetric RT-LAMP, to diagnose the RdRp and ORF8 genes, respectively, in oro-nasopharyngeal swabs. Due to the high sequence diversity of ORF8 in SARS-CoV and SARS-CoV-2, it has been identified as a suitable target for virus detection. The RT-LAMP method was also carried out directly on heat-treated swab samples. The strip tests were made using gold nanoparticles and combined with the RT-LAMP for further analysis. Results: The results showed that the isothermal amplification method had a sensitivity of 95 % (95 % C.I.: 86.08 %-98.96 %) and a specificity of 75 % (95 % C.I.: 19.41 %-99.37 %). The RT-LAMP-LFA method was able to distinguish positive and negative samples with 100 % sensitivity (95 % C.I.: 91.96-100) and 77.27 % specificity (95 % C.I.: 54.63-92.18). This method only required heating swab samples for 10 min at 65 °C before the RT-LAMP reaction. Conclusion: By utilizing the RT-LAMP in combination with the LFA, it is possible to diagnose SARS-CoV-2 rapidly without the need for RNA extraction. The entire process from sample collection to test interpretation takes only 75-90 min, and the results can be interpreted by untrained individuals with the naked eye. By employing the ORF8 gene as a diagnostic target and eliminating the need for RNA extraction, the direct RT-LAMP-LFA method achieves a significant breakthrough that was not previously reported.
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