Evidence map›Paper›PMID 39553427›Full record

ArticleBioengineering & translational medicine2024

Model acetylcholinesterase-Fc fusion glycoprotein biotechnology system for the manufacture of an organophosphorus toxicant bioscavenging countermeasure.

Thomas G Biel, Talia Faison, Alicia M Matthews, Uriel Ortega-Rodriguez, Vincent M Falkowski, Edward Meek, Xin Bush, Matthew Flores, Sarah Johnson, Wells W Wu and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineering & translational medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Thomas G BielOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.ORCID https://orcid.org/0000-0001-8592-6184
Talia FaisonOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Alicia M MatthewsOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Uriel Ortega-RodriguezOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Vincent M FalkowskiOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Edward MeekDepartment of Comparative Biomedical Sciences, Center for Environmental Health Sciences College of Veterinary Medicine, Mississippi State University Mississippi State Mississippi USA.
Xin BushOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Matthew FloresOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Sarah JohnsonOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Wells W WuFacility for Biotechnology Resources Center for Biologics Evaluation and Research, United States Food and Drug Administration Silver Spring Maryland USA.
Mari LehtimakiOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Rong-Fong ShenFacility for Biotechnology Resources Center for Biologics Evaluation and Research, United States Food and Drug Administration Silver Spring Maryland USA.
Cyrus AgarabiOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
V Ashutosh RaoOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.
Janice E ChambersDepartment of Comparative Biomedical Sciences, Center for Environmental Health Sciences College of Veterinary Medicine, Mississippi State University Mississippi State Mississippi USA.
Tongzhong JuOffice of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration Silver Spring Maryland USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Organophosphate (OP) toxicants remain an active threat to public health and to warfighters in the military. Current countermeasures require near immediate administration following OP exposure and are reported to have controversial efficacies. Acetylcholinesterase (AChE) fused to the human immunoglobulin 1 (IgG1) Fc domain (AChE-Fc) is a potential bioscavenger for OP toxicants, but a reproducible AChE-Fc biomanufacturing strategy remains elusive. This report is the first to establish a comprehensive laboratory-scale bioprocessing strategy that can reproducibly produce AChE-Fc and AChE(W86A)-Fc which is a mutated AChE protein with reduced enzymatic activity. Characterization studies revealed that AChE-Fc and AChE(W86A)-Fc are

Indexed as

acetylcholinesterase fusion proteinbiomanufacturingbioscavengerglycosylationorganophosphorus toxicant

Identifiers

PMID39553427
PMCPMC11561780

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.