Evidence map›Paper›PMID 39551756›Full record

ArticleBMC veterinary research2024

Comparative study of immunoassays, a microelectromechanical systems-based biosensor, and RT-QuIC for the diagnosis of chronic wasting disease in white-tailed deer.

Estela Kobashigawa, Sura A Muhsin, Amjed Abdullah, Keara Allen, Emily A Sinnott, Michael Z Zhang, Sherri Russell, Mahmoud Almasri, Shuping Zhang

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Estela Kobashigawa *Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 901 E. Campus Loop, Columbia, MO, USA.
Sura A Muhsin *Department of Electrical Engineering and Computer Science, University of Missouri, Columbia, MO, USA.
Amjed AbdullahDepartment of Electrical Engineering and Computer Science, University of Missouri, Columbia, MO, USA.
Keara AllenVeterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 901 E. Campus Loop, Columbia, MO, USA.
Emily A SinnottMissouri Department of Conservation, 2901 W Truman Blvd, Jefferson City, MO, USA.
Michael Z ZhangVeterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 901 E. Campus Loop, Columbia, MO, USA.
Sherri RussellMissouri Department of Conservation, 2901 W Truman Blvd, Jefferson City, MO, USA.
Mahmoud AlmasriDepartment of Electrical Engineering and Computer Science, University of Missouri, Columbia, MO, USA.
Shuping ZhangVeterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 901 E. Campus Loop, Columbia, MO, USA. zhangshup@missouri.edu.

Funding

Missouri Department of Conservation 00069940
6 · The paper itself

Abstract

backgroundChronic wasting disease (CWD) is a fatal transmissible spongiform encephalopathy in cervids. The disease is caused by a pathogenic prion, namely PrP

resultsBoth CWD Ag-ELISA and TeSeE ELISA correctly identified all CWD + and CWD- samples. A greater intra-assay coefficient of variation (CV) in S/P ratios was observed for the TeSeE ELISA (16.52%), compared to CWD Ag-ELISA (9.49%). However, the high CV did not affect the qualitative results of triplicate assays when the corresponding manufacturer's cutoff was used. The MEMS biosensor not only correctly identified all CWD + and CWD- RPLN samples, but also demonstrated a 100% detection rate for all CWD + samples at dilutions from 10

conclusionsThe two immunoassays (CWD Ag-ELISA and TeSeE ELISA) performed comparably on white-tailed deer RPLN samples. MEMS biosensor is a reliable portable tool for CWD diagnosis and RT-QuIC can be used for routine testing of CWD if appropriate testing parameters and interpretive criteria are applied.

Indexed as

Biosensing TechniquesDeerEnzyme-Linked Immunosorbent AssayWasting Disease, ChronicAnimalsImmunoassayMicro-Electrical-Mechanical SystemsSensitivity and SpecificityCWDdeerDielectrophoresisELISAIHCMEMS biosensorRapid detectionRPLNRT-QuICSensitivitySpecificityS/P value

Identifiers

PMID39551756
PMCPMC11571681

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.