Evidence map›Paper›PMID 39550025›Full record

ArticleDevelopmental biology2025

Maternal and zygotic contributions to H3K4me1 chromatin marking during germ layer formation.

Kitt D Paraiso, Ira L Blitz, Ken W Y Cho

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kitt D ParaisoDevelopmental and Cell Biology, University of California, Irvine, CA, USA; Center for Complex Biological Systems, University of California, Irvine, CA, USA.
Ira L BlitzDevelopmental and Cell Biology, University of California, Irvine, CA, USA.
Ken W Y ChoDevelopmental and Cell Biology, University of California, Irvine, CA, USA; Center for Complex Biological Systems, University of California, Irvine, CA, USA. Electronic address: kwcho@uci.edu.

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
Deciphering the gene regulatory network controlling vertebrate endodermal fatesR01HD073179 · NICHD · UNIVERSITY OF CALIFORNIA-IRVINE · PI CHO, KEN W.Y., MJOLSNESS, ERIC D · 2013 to 2017
$2.9M
Zygotic Genome Activation by Maternal Transcription Factors and Epigenetic ModifiersR35GM139617 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Ken W.Y. Cho · 2021 to 2026
$2.6M
Mechanisms of Foxh1 pioneer factor action during mesendoderm developmentR01GM126395 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI CHO, KEN W.Y. · 2017 to 2020
$1.6M
PacBio RS Single Molecule, Real-Time (SMRT) DNA SequencerS10OD010794 · OD · UNIVERSITY OF CALIFORNIA-IRVINE · PI SANDMEYER, SUZANNE · 2012 to 2012
$600k
High-Throughput DNA SequencerS10OD021718 · OD · UNIVERSITY OF CALIFORNIA-IRVINE · PI SANDMEYER, SUZANNE · 2016 to 2016
$600k
High Throughput DNA SequencerS10RR025496 · NCRR · UNIVERSITY OF CALIFORNIA-IRVINE · PI SANDMEYER, SUZANNE · 2009 to 2009
$500k
NCI NIH HHS P30 CA062203NCRR NIH HHS S10 RR025496NICHD NIH HHS R01 HD073179NIGMS NIH HHS R01 GM126395NIGMS NIH HHS R35 GM139617NIH HHS S10 OD010794NIH HHS S10 OD021718
6 · The paper itself

Abstract

An early step in triploblastic embryo differentiation is the formation of the three germ layers. Maternal pioneer transcription factors (TFs) bind to embryonic enhancers before zygotic genome activation, initiating germ layer specification. While maternal TFs' role in establishing epigenetic marks is known, how early pluripotent cells gain spatially restricted epigenetic identities remains unclear. We show that by the early gastrula stage, H3K4me1-marked regions become distinct in each germ layer, with certain chromatin regions forming high density H3K4me1 marked regions (HDRs). Genes associated with these HDRs are more robustly expressed compared to those associated with low density H3K4me1 marked regions (LDRs) in the genome. This process is driven by the sequential actions of maternal and zygotic factors. Knockdown of key maternal endodermal TFs (Otx1, Vegt and Foxh1) leads to a loss of endodermal H3K4me1 marks in endoderm, with a concurrent emergence of ectodermal and mesodermal marks, indicating a shift in chromatin state. This work highlights the importance of coordinated activities of maternal and zygotic TFs in defining the regionally-resolved and dynamic process of chromatin modification conferred by H3K4me1 in the early Xenopus embryo.

Indexed as

ChromatinGene Expression Regulation, DevelopmentalHistonesXenopus laevisXenopus ProteinsZygoteAnimalsEndodermEpigenesis, GeneticFemaleGastrulaGerm LayersLysineTranscription FactorsChromatinH3K4me1HistonesLysineTranscription FactorsXenopus ProteinsCanalizationEpigeneticsGene expressionHistone modificationRobustnessSuper enhancersTranscription factorsZygotic genome activation

Identifiers

PMID39550025
PMCPMC11796633

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.