Evidence map›Paper›PMID 39549467›Full record

ArticleVision research2025

Intravitreal AAV2 gene delivery to feline retinal ganglion cells.

Kazuya Oikawa, J Seth Eaton, Julie A Kiland, Odalys Torné, Virginia Mathu, Robert W Nickells, Gillian J McLellan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vision research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kazuya OikawaDepartment of Ophthalmology & Visual Sciences, University of Wisconsin-Madison, Madison, WI, United States; Department of Surgical Sciences, University of Wisconsin-Madison, Madison, WI, United States; McPherson Eye Research Institute, Madison, WI, United States.
J Seth EatonDepartment of Surgical Sciences, University of Wisconsin-Madison, Madison, WI, United States; McPherson Eye Research Institute, Madison, WI, United States.
Julie A KilandDepartment of Ophthalmology & Visual Sciences, University of Wisconsin-Madison, Madison, WI, United States.
Odalys TornéDepartment of Ophthalmology & Visual Sciences, University of Wisconsin-Madison, Madison, WI, United States; Department of Surgical Sciences, University of Wisconsin-Madison, Madison, WI, United States; McPherson Eye Research Institute, Madison, WI, United States.
Virginia MathuDepartment of Ophthalmology & Visual Sciences, University of Wisconsin-Madison, Madison, WI, United States; Department of Surgical Sciences, University of Wisconsin-Madison, Madison, WI, United States; McPherson Eye Research Institute, Madison, WI, United States.
Robert W NickellsDepartment of Ophthalmology & Visual Sciences, University of Wisconsin-Madison, Madison, WI, United States; McPherson Eye Research Institute, Madison, WI, United States.
Gillian J McLellanDepartment of Ophthalmology & Visual Sciences, University of Wisconsin-Madison, Madison, WI, United States; Department of Surgical Sciences, University of Wisconsin-Madison, Madison, WI, United States; McPherson Eye Research Institute, Madison, WI, United States. Electronic address: gillian.mclellan@wisc.edu.

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Justine Yang Bruce · 1985 to 2026
$142.6M
University of Wisconsin Institute for Clinical and Translational ResearchUL1TR002373 · NCATS · UNIVERSITY OF WISCONSIN-MADISON · PI ELIZABETH S BURNSIDE, Allan R. Brasier · 2017 to 2026
$75.9M
UW Vision Research Core - Administrative CoreP30EY016665 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI AKIHIRO IKEDA · 2005 to 2026
$12.6M
Therapeutic Inhibition of Optic Nerve Head Gliosis and Fibrosis in GlaucomaR01EY027396 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI MCLELLAN, GILLIAN JANE · 2017 to 2021
$2.0M
Targeting BAX as a Therapeutic for Protection of Retinal Ganglion CellsR01EY030123 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI NICKELLS, ROBERT W · 2019 to 2022
$1.9M
Enhancing Basic and Translational Vision Research Using AnimalsS10OD026957 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI BRANDT, CURTIS R · 2019 to 2019
$529k
Spectral domain optical coherence tomography instrument for multimodal imagingS10OD018221 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI MCLELLAN, GILLIAN JANE · 2014 to 2014
$210k
Automated Tissue MicroarrayerS10OD023526 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI MATKOWSKYJ, KRISTINA A. · 2018 to 2018
$184k
NCATS NIH HHS UL1 TR002373NCI NIH HHS P30 CA014520NEI NIH HHS P30 EY016665NEI NIH HHS R01 EY027396NEI NIH HHS R01 EY030123NIH HHS S10 OD018221NIH HHS S10 OD023526NIH HHS S10 OD026957
6 · The paper itself

Abstract

Effective strategies for the neuroprotection and preservation of retinal ganglion cells (RGCs) remain elusive in the management of glaucoma. A spontaneous genetic model of glaucoma has been identified in cats and extensively characterized as a viable translational model, with eye size and anatomy similar to humans. In this study we sought to establish initial proof of concept for gene delivery to feline RGCs via intravitreal injection of AAV2 in normal cats. Pre-retinal, posterior vitreal injection of AAV2/2-CMV-GFP, was performed overlying the area centralis in 5 adult cats. Immunosuppressive oral prednisolone was administered perioperatively and gradually tapered over 6-10wks post-injection. Ophthalmic examination was performed pre- and post-injection. The GFP reporter expression and morphological effects of viral transduction on the retina were monitored in vivo using confocal scanning laser ophthalmoscopy (cSLO) and optical coherence tomography (OCT), respectively (Spectralis OCT-HRA, Heidelberg), at 1-2wk intervals over 6-10wks. Full-field electroretinograms (ERG) and visual evoked potentials (VEP) were recorded at baseline and post-injection. Retinas were examined by histology and immunolabeling for the RGC marker RBPMS and Müller cell and astrocyte marker SOX9, and GFP expression was examined in the retina, optic nerve (ON), optic tract and lateral geniculate nucleus (LGN). GFP

Indexed as

DependovirusElectroretinographyIntravitreal InjectionsRetinal Ganglion CellsTomography, Optical CoherenceAnimalsCatsDisease Models, AnimalEvoked Potentials, VisualGenetic TherapyGenetic VectorsGene Transfer TechniquesGreen Fluorescent ProteinsGreen Fluorescent ProteinsAdeno-Associated Virus 2CatGene therapyGlaucomaIntravitreal injectionLarge animal modelRetinal Ganglion Cells

Identifiers

PMID39549467
PMCPMC11658774

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.