Evidence map›Paper›PMID 39549085›Full record

ArticleCellular and molecular life sciences : CMLS2024

rTM reprograms macrophages via the HIF-1α/METTL3/PFKM axis to protect mice against sepsis.

Chen Yao, Hanyong Zhu, Binbin Ji, Hui Guo, Zimeng Liu, Ni Yang, Qi Zhang, Kangning Hai, Chenbo Gao, Jie Zhao and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Review
  10. Review
  11. Semaglultide targets Spp1Journal of neuroinflammation · 2025
    Article
  12. Review
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Chen Yao *Jiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Hanyong Zhu *Jiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Binbin JiJiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Hui GuoJiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Zimeng LiuNational Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Ni YangNational Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Qi ZhangNational Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Kangning HaiNational Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Chenbo GaoNational Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Jie ZhaoJiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Xueqin LiJiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Rongqing LiDepartment of Medical Genetics and Prenatal Diagnosis, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, 225399, Jiangsu, China.
Xin ChenDepartment of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 310058, Zhejiang, China.
Fandong MengDepartment of Endocrinology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Xiucheng PanDepartment of Infectious Disease, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Chunling FuDepartment of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Wanpeng ChengJiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Fuxing DongPublic Experimental Research Center, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Jing YangJiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China. jingyang@xzhmu.edu.cn.
Yuchen PanJiangsu International Laboratory of Immunity and Metabolism, Jiangsu Province Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China. panyuchen@xzhmu.edu.cn.ORCID http://orcid.org/0000-0001-9260-4562
Takayuki IkezoeDepartment of Hematology, Fukushima Medical University, Fukushima, 960-1296, Japan.

Funding

An Open Competition Grant of Xuzhou Medical University JBGS202202The Foundation for Key Program of Universities of Jiangsu Province 23KJA310011The National Natural Science Foundation of Jiangsu Province BK20201461The Postgraduate Research & Practice Innovation Program of Jiangsu Province KYCX22_2874The science and technology project of Xuzhou City KC23027The Scientific Starting Grants for Talented Early-career Researchers RC20552220The Zhejiang Medical and Health Science and Technology Project 2024KY501
6 · The paper itself

Abstract

The metabolic reprogramming of macrophages is a potential therapeutic strategy for sepsis treatment, but the mechanism underlying this reprogramming remains unclear. Since glycolysis can drive macrophage phenotype switching, the rate-limiting enzymes in glycolysis may be key to treating sepsis. Here, we found that, compared with other isoenzymes, the expression of 6-phosphofructokinase, muscle type (PFKM) was the most upregulated in monocytes from septic patients. Recombinant thrombomodulin (rTM) treatment downregulated the protein expression of PFKM in macrophages. Both rTM treatment and Pfkm knockout protected mice from sepsis and reduced the production of the proinflammatory cytokines IL-1β, IL-6, TNF-α, and IL-27, whereas PFKM overexpression increased the production of these cytokines. Mechanistically, rTM treatment inhibited glycolysis in macrophages by decreasing PFKM expression in a hypoxia-inducible factor-1α (HIF-1α)-dependent manner. HIF-1α overexpression increased methyltransferase-like 3 (METTL3) expression, elevated the m

Indexed as

Hypoxia-Inducible Factor 1, alpha SubunitMacrophagesSepsisAnimalsCellular ReprogrammingCytokinesFemaleGlycolysisHumansMaleMethyltransferasesMiceMice, Inbred C57BLMice, KnockoutCytokinesHypoxia-Inducible Factor 1, alpha SubunitMethyltransferases6-phosphofructokinaseGlycolysisMacrophagesMethyltransferase-like 3 (METTL3)Muscle type (PFKM)SepsisThrombomodulin

Identifiers

PMID39549085
PMCPMC11569104

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.