ArticlePlant methods2024
Establishment of callus induction and plantlet regeneration systems of Peucedanum Praeruptorum dunn based on the tissue culture method.
Article in Plant methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed.
- Identification of Potential Candidate Dwarf Genes in Maize by BSA-Seq and Transcriptomic Analyses.Plants (Basel, Switzerland) · 2026Article
- Stem Cells in Cosmetic Applications: Advancements, Challenges, and Future Directions.Journal of cosmetic dermatology · 2026Review
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- In vitro bioactive metabolite production and plant regeneration of medicinal plant Ligusticum chuanxiong Hort.BMC plant biology · 2026Article
- Quality formation inFrontiers in plant science · 2026Review
- Physiological and Biochemical Responses ofLife (Basel, Switzerland) · 2025Article
- Establishment of a refined somatic embryogenesis protocol and light-spectrum-based acclimatization in Caladium bicolor 'White'.BMC plant biology · 2025Article
- Evolutionary Insight and Expression Pattern of WUSCHEL-Related Homebox Genes ofFood science & nutrition · 2025Article
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Abstract
backgroundPeucedanum praeruptorum Dunn has typical stacked umbels and medicinal value; however, the lack of an effective tissue culture system for P. praeruptorum has limited the large-scale propagation of its seedlings.
resultsWe systematically established an in vitro regeneration system for P. praeruptorum using young leaves and stems as explants. Tissue culture plantlets were successfully obtained within 123 and 90 d of somatic embryogenesis and organogenesis, respectively. Combined plant growth regulators (PGRs) were optimized to promote efficient plant regeneration at each stage of the culture process. Specifically, embryogenic callus induction was superior in Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 6-benzyladenine (BA) and 2.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). For somatic embryonic development, the highest differentiation rates were achieved using BA, 2,4-D, and 6-furfuryl aminopurine (6-KT). Induction of organogenesis resulted in the highest differentiation rates and proliferation coefficients of buds in MS medium supplemented with BA and α-naphthaleneacetic acid (NAA). Moreover, regeneration of P. praeruptorum seedlings was achieved by adjusting the BA and indole-3-butyric acid (IBA) concentrations in 1/2 MS medium.
conclusionOur results provide a technical system for the rapid propagation of P. praeruptorum, which can facilitate germplasm improvement, resource conservation, and further genetic transformation of Peucedanum species.
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