Evidence map›Paper›PMID 39548497›Full record

ArticleBMC biology2024

Building an eDNA surveillance toolkit for invasive rodents on islands: can we detect wild-type and gene drive Mus musculus?

Antoinette J Piaggio, Luke Gierus, Daniel R Taylor, Nick D Holmes, David J Will, Neil J Gemmell, Paul Q Thomas

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Antoinette J PiaggioU.S. Department of Agriculture, Animal Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, Fort Collins, CO, USA. toni.j.piaggio@usda.gov.
Luke GierusSchool of Biomedicine and Robinson Research Institute, University of Adelaide, Adelaide, Australia.
Daniel R TaylorU.S. Department of Agriculture, Animal Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, Fort Collins, CO, USA.
Nick D HolmesThe Nature Conservancy, Santa Cruz, CA, USA.
David J WillIsland Conservation, Santa Cruz, CA, USA.
Neil J GemmellDepartment of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
Paul Q ThomasSchool of Biomedicine and Robinson Research Institute, University of Adelaide, Adelaide, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundInvasive management strategies range from preventing new invasive species incursions to eliminating established populations, with all requiring effective monitoring to guide action. The use of DNA sampled from the environment (eDNA) is one such tool that provides the ability to surveille and monitor target invasive species through passive sampling. Technology being developed to eliminate invasive species includes genetic biocontrol in the form of gene drive. This approach would drive a trait through a population and could be used to eliminate or modify a target population. Once a gene drive organism is released into a population then monitoring changes in density of the target species and the spread of the drive in the population would be critical.

resultsIn this paper, we use invasive Mus musculus as a model for development of an eDNA assay that detects wild-type M. musculus and gene drive M. musculus. We demonstrate successful development of an assay where environmental samples could be used to detect wild-type invasive M. musculus and the relative density of wild-type to gene drive M. musculus.

conclusionsThe development of a method that detects both wild-type M. musculus and a gene drive M. musculus (t

Indexed as

DNA, EnvironmentalGene Drive TechnologyIntroduced SpeciesAnimalsIslandsMiceDNA, EnvironmentalCRISPR/CasEnvironmental DNA (eDNA)Gene driveInvasive speciesMus musculus

Identifiers

PMID39548497
PMCPMC11566076

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.